Goat Anti-Mouse IgG2c heavy chain (ab97251)

Overview

  • Product name
    Goat Anti-Mouse IgG2c heavy chain
    See all IgG2c secondary antibodies
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins.
  • Tested applications
    Suitable for: IHC-P, ELISA, WB, ICCmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/2000.
ELISA 1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000)
WB 1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)
ICC Use at an assay dependent dilution.

References

ab97251 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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