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By immunoelectrophoresis and ELISA, the antiserum reacts specifically with mouse IgG2c in C57BL/6, SJL, C57BL/10, CB20, C57BL/6 by Balb/C crosses and pools of serum of outbred mice obtained from several commercial sources. No antibody was detected against immunoglobulin light chains, other IgG subclasses or non-immunogloublin serum proteins.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Antiserum was solid phase adsorbed to ensure subclass specificity to IgG2c. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/2000.
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000)
1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000)
Use at an assay dependent dilution.
References for Goat Anti-Mouse IgG2c heavy chain (ab97251)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"