Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed (ab47844)

Overview

  • Product nameGoat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed
    See all IgG + IgM secondary antibodies
  • Target speciesMouse
  • SpecificityThis antibody reacts with Mouse IgG and IgM heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, IHC-Pmore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Full length IgG and IgM molecules (Mouse)

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes10-20 BAC (Biotinamidocaproate N-Hydroxysuccinimide Ester) molecules per IgG molecule
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/20000 - 1/100000.
WB 1/2000 - 1/10000.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent dilution.

Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed images

  • Ab47844 was used at dilution 1/1000 with the primary antibody ab24575 in ICC/IF. See the review on ab24575.

References for Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed (ab47844)

ab47844 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"