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Product name Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed See all IgG + IgM secondary antibodies
Target species Mouse
Specificity This antibody reacts with Mouse IgG and IgM heavy and light chains. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Biotin, Mouse IgG and Mouse IgM.
Tested applications Suitable for:
ELISA, WB, ICC/IF, IHC-P more details
Minimal cross-reactivity Cow, Horse, Human
To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
Full length IgG and IgM molecules (Mouse)
Conjugation Biotin Properties
Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage buffer Preservative: 0.01% Sodium Azide
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes 10-20 BAC (Biotinamidocaproate N-Hydroxysuccinimide Ester) molecules per IgG molecule
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20000 - 1/100000.
1/2000 - 1/10000.
1/1000 - 1/5000.
Use at an assay dependent dilution.
Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed images
Immunocytochemistry/ Immunofluorescence - Goat polyclonal Secondary Antibody to Mouse IgG+IgM - H&L (Biotin), pre-adsorbed (ab47844)
Ab47844 was used at dilution 1/1000 with the primary antibody ab24575 in ICC/IF. See the review on ab24575.
References for Goat Anti-Mouse IgG+IgM H&L (Biotin) preadsorbed (ab47844)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"