Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed (ab47827)

Overview

  • Product name
    Goat Anti-Mouse IgG+IgM H&L (HRP) preadsorbed
    See all IgG + IgM secondary antibodies
  • Target species
    Mouse
  • Specificity
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase, Mouse IgG and Mouse IgM.
  • Tested applications
    Suitable for: ICC/IF, ELISA, WB, IHC-P, IHC-Fr, Electron Microscopymore details
  • Minimal
    cross-reactivity

    Cow, Horse, Human more details
  • Immunogen

    Full length native proteins (purified) (Mouse)

  • Conjugation
    HRP

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption (s) to remove any unwanted reactivities.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/2500.
ELISA 1/5000 - 1/40000.
WB 1/2000 - 1/10000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Electron Microscopy Use at an assay dependent dilution.

Images

  • Ab47827 was used at dilution 1/500 with the primary antibody ab2869 in IHC-P. See the review on ab2869.

References

This product has been referenced in:
  • Wang SK  et al. Fam83h null mice support a neomorphic mechanism for human ADHCAI. Mol Genet Genomic Med 4:46-67 (2016). WB . Read more (PubMed: 26788537) »
  • Ji Q  et al. Intrathecal injection of phosphodiesterase 4B-specific siRNA attenuates neuropathic pain in rats with L5 spinal nerve ligation. Mol Med Rep 13:1914-22 (2016). Read more (PubMed: 26706904) »

See all 4 Publications for this product

Customer reviews and Q&As

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I am happy to confirm that ab47827 (Goat anti-Mouse IgG + IgM H&L (HRP) secondary antibody) is a suitable secondary antibody for ab3227 and ab22045.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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