Goat Anti-Mouse IgM mu chain (Agarose) (ab65867)

Overview

  • Product nameGoat Anti-Mouse IgM mu chain (Agarose)
    See all IgM secondary antibodies
  • Target speciesMouse
  • SpecificitySpecificity for the mu-chain of mouse IgM is determined by Ouchterlony Double Diffusion (ODD), prior to agarose bead coupling. The antibody preparation is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses) and IgM myeloma proteins. Identity and purity of the antibody is established by immunoelectrophoresis (IEP), prior to agarose bead coupling. Electrophoresis of the product followed by diffusion against anti-goat IgG and anti-goat whole serum results in single arcs of precipitation in the gamma region.
  • Immunogen

    Mouse IgM

  • ConjugationAgarose

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituent: 2.9% Sodium chloride
  • Concentration information loading...
  • PurityIgG fraction
  • Conjugation notesThe IgG fraction of goat anti-mouse IgM (mu-chain specific) antiserum is covalently attached to cyanogen bromide activated cross-linked beaded agarose. Five to ten milligrams of IgG fraction is bound per milliliter of resin. After equilibration, a minimum of 0.4 mg of mouse IgM can be bound and eluted per milliliter of packed resin.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesProcedure
    A two milliliter column of antibody-agarose is prepared using four milliliters of the antibody-agarose suspension. The column is equilibrated in 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M NaCl (PB). The antigen solution to be bound is applied slowly and followed by a PB wash. Unbound effluent fractions are collected and assayed for protein content (Lowry). The column is then stripped by washing with 0.1 M glycine, 0.15 M NaCl, pH 2.4, or 0.5 M acetic acid, 0.15 M NaCl, pH 2.4. Fractions containing protein are collected, brought to neutral pH and assayed for protein content (Lowry).

    Regeneration
    Goat Anti-Mouse IgM-Agarose may be regenerated and used for future adsorptions. Strip the agarose with ten column volumes of 0.1 M glycine, 0.15 M sodium chloride, pH 2.4, or 0.5 M acetic acid, 0.15 M sodium chloride, pH 2.4, then wash with 0.01 M sodium phosphate buffer, pH 7.2, containing 0.5 M sodium chloride (PB).
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65867 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent dilution.

References for Goat Anti-Mouse IgM mu chain (Agarose) (ab65867)

ab65867 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam technical services regarding ab65867 - Anti-Mouse IgM.   I can confirm that this product has been tested for affinity purification only and we haven't tested it for immunoprecipitation specifically. As the principle i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"