Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647) (ab150123)
Key features and details
- Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647)
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Mouse IgM mu chain (Alexa Fluor® 647)
See all IgM secondary antibodies -
Host species
Goat -
Target species
Mouse -
Tested applications
Suitable for: IHC-Fr, ICC/IF, Flow Cyt, ELISA, IHC-Pmore details -
Immunogen
The details of the immunogen for this antibody are not available.
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Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C. Store In the Dark. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 23% Glycerol (glycerin, glycerine), PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150123 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 2 µg/ml.
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Flow Cyt | (1) |
1/2000.
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
Flow Cyt
1/2000. |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Images
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ICC/IF image of ab20346 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab20346, 1/500) overnight at +4°C. The secondary antibody (red) was ab150123 used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab150123 has been referenced in 15 publications.
- Zhang B et al. Proximity-enabled covalent binding of IL-2 to IL-2Rα selectively activates regulatory T cells and suppresses autoimmunity. Signal Transduct Target Ther 8:28 (2023). PubMed: 36690610
- Liu YS et al. Accumulated precursors of specific GPI-anchored proteins upregulate GPI biosynthesis with ARV1. J Cell Biol 222:N/A (2023). PubMed: 36828365
- Li W et al. Single-cell RNA-seq of heart reveals intercellular communication drivers of myocardial fibrosis in diabetic cardiomyopathy. Elife 12:N/A (2023). PubMed: 37010266
- Laudato JA et al. Impact of prior alcohol use on the subsequent development of cancer cachexia in male and female mice. Alcohol Clin Exp Res (Hoboken) 47:1271-1282 (2023). PubMed: 37208964
- Tatsukawa T et al. NG2-positive pericytes regulate homeostatic maintenance of slow-type skeletal muscle with rapid myonuclear turnover. Stem Cell Res Ther 14:205 (2023). PubMed: 37592340