Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)

Overview

  • Product nameGoat Anti-Mouse IgM mu chain (DyLight® 488)See all IgM secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (DyLight® 488)
  • Target speciesMouse
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm

Properties

Applications

Our Abpromise guarantee covers the use of ab97007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.

Goat Anti-Mouse IgM mu chain (DyLight® 488) images

  • Overlay histogram showing HeLa cells stained with ab130935 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab130935, 1µg/1x10^6 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgM (mu chain) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgM [ICIGM] (ab91545, 2µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 20 min used under the same conditions.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Mouse IgM mu chain (DyLight® 488) (ab97007)

ab97007 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab97007.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"