Overview

  • Product name
    Goat Anti-Mouse IgM mu chain (HRP)
    See all IgM secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Mouse IgM - mu chain (HRP)
  • Target species
    Mouse
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgM. Cross reactivity with other immunoglobulins and light chains is less than 0.1%.
  • Tested applications
    Suitable for: ELISA, WB, ICC, IHC-Pmore details
  • Conjugation
    HRP

Properties

Applications

Our Abpromise guarantee covers the use of ab97230 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminiscence: 1/5000 - 1/25000)
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.

Images

  • IHC image of Hsp70 staining in human lung formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab5442, 1/2000 dilution overnight at +4°C. An HRP-conjugated secondary (ab97230, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

References

This product has been referenced in:
  • Frenkel D  et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). Read more (PubMed: 27403737) »
  • Morash AJ  et al. Pass the salt: physiological consequences of ecologically relevant hyposmotic exposure in juvenile gummy sharks (Mustelus antarcticus) and school sharks (Galeorhinus galeus). Conserv Physiol 4:cow036 (2016). Read more (PubMed: 27757235) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your interest in our secondary antibodies. I will answer your questions as best I can now but cross-reactivity of the mouse anti-human IgG isotype antibodies will require data from tests which I do not have at hand. I will forward these t...

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Thank you for contacting us.

I am sorry but I do not fully understand what the customer is hoping to achieve and have thereforefound it difficult to answer your question. How are they testing the antibodies with nanoparticles?

It is...

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Thank you for your inquiry. Indeed, antibodies as most (unmodified) proteins have a carboxy group (-COOH) at their C-terminus, as you indicated. They also have an amine group (-NH2) at their N-terminus. Furthermore, antibodies contain amino acids w...

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Thank you for your enquiry. I am sorry the customer has some concerns regarding the results of their experiment. We would not recommend to store the antigen-antibody samples in distilled water. The sample and the antibody binding are likely to be m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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