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Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed (ab7117)

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    Overview

    Product name

    Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed
    See all IgY products (89) ...

    Target species

    Chicken

    Tested applications

    ICC/IF, Dot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

    Minimal
    cross-reactivity


    Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat, Sheepmore details

    Immunogen

    Full length native chicken IgY (purified).

    Properties

    Form

    Liquid

    Storage instructions

    Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Storage buffer

    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2

    Concentration

    Concentration information loading...

    Purity

    IgY fraction

    Purification notes

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using chicken IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Conjugation notes

    Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule

    Clonality

    Polyclonal

    Research areas

    Secondary antibodies >> anti-Chicken >> IgY >> Biotin

    Applications

    Show applications key

    Our Abpromise guarantee covers the use of ab7117 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • ICC/IF

       

    • Dot Blot

       

    • ELISA

       

    • IHC-P

       

    • IHC-Fr

       

    • Immunomicroscopy

       

    • WB

       

    Application notes

    Dot: Use at an assay dependant dilution.
    ELISA: 1/8000 - 1/36000.
    IHC-P: Use at an assay dependant dilution.
    IHC-Fr: Use at an assay dependant dilution.
    IM: Use at an assay dependant dilution.
    WB: Use at an assay dependant dilution.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    References for Goat polyclonal Secondary Antibody to Chicken IgY - H&L (Biotin), pre-adsorbed (ab7117)

    ab7117 has not yet been referenced specifically in any publications.

    Publishing research using ab7117? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"