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Products:Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Overview
Product name
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin)
See all IgG products (1691) ...
Target species
Rabbit
Tested applications
WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Fr, IHC-Pmore details
Immunogen
Rabbit IgG whole molecule
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2
Concentration
Concentration information loading...
Purity
IgG fraction
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Conjugation notes
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
Clonality
Polyclonal
Research areas
Secondary antibodies >> anti-Rabbit >> IgG >> Biotin
Immunology >> Immunoglobulins >> Heavy Chain >> IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6720 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/5000 - 1/20000.WB: 1/5000 - 1/20000.
ELISA: 1/50000 - 1/200000.
IM: Use at an assay dependent concentration.
Dot: Use at an assay dependent concentration.
ICC/IF
ICC/IF: 1/1000 - 1/5000.ICC/IF: 1/1000 - 1/5000.
IHC-Fr: Use at an assay dependent concentration.
IHC-P: 1/200(PubMed: 17041957)
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG antibody - H&L (ab6720)
ab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.
This image is courtesy of an anonymous Abreview
Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab6720)
Ab6720 was used at dilution 1/100 with the primary antibody ab15000 in IHC-Fr. See the review on ab15000.
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab6720)
This product has been referenced in:
- Swedin Let al. Prostaglandin modulation of airway inflammation and hyperresponsiveness in mice sensitized without adjuvant. Prostaglandins Other Lipid Mediat 92:44-53 (2010). IHC-P.Read more (PubMed: 20214998) »
- McEgan Ret al. Concentration and detection of Salmonella in mung bean sprout spent irrigation water by use of tangential flow filtration coupled with an amperometric flowthrough enzyme-linked immunosorbent assay. J Food Prot 72:591-600 (2009).Read more (PubMed: 19343949) »
See all 7 publications for this product
Publishing research using ab6720? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"