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Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

Submit an Abreview Q&A (80)Specific References (53)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsIHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Fr more details
  • Immunogen

    Rabbit IgG, whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • General notesMany of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/1000.
WB WB: 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).

Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.
ELISA ELISA: 1/120000.
Immunomicroscopy IM: Use at an assay dependent dilution.
Dot Blot Dot: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/1000 - 1/5000.
IHC-Fr IHC-Fr: 1/1000.

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) images

  • Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Ab6721 was used with the primary antibody ab8227 in WB. See the review on ab8227.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.
  • Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
    Ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.

References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)

This product has been referenced in:
  • Mancek-Keber M  et al. MARCKS as a negative regulator of lipopolysaccharide signaling. J Immunol 188:3893-902 (2012). Read more (PubMed: 22427633) »
  • Benneyworth MA  et al. Failure of NMDA receptor hypofunction to induce a pathological reduction in PV-positive GABAergic cell markers. Neurosci Lett 488:267-71 (2011). WB . Read more (PubMed: 21094213) »

See all 53 Publications for this product

Publishing research using ab6721 ? Please let us know so that we can cite the reference in this datasheet

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Displaying 1 - 10 of 80 results for Abreviews and Q&A

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Inquiry: Dear Sir, I was just checking the dilution I should use for IHC-P in the datasheet it's written 1:1000 in all the reviews it's written 1:100 so which one is correct? as it is 10 time different concentration thanks for help Regards

Thank you for your enquiry regarding ab6721.

The dilution factor on the datasheet only a recommendation for starting. The actual dilutions need to be optimized by the scientist since it depends on many factors such expression levels of the targe...

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thank you for the offering of free of charge replacement. Please let me know what I have to do and the information that you need for this.

Thank you so much!

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has ...

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thank you for your quick answer.
The order number of this product is #1072465. This antibody was stored at -20˚C in aliquots and I avoided freeze / thaw cycles all the time (I have aliquoted and never refreeze it). I have seen the same binding pattern...

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Thank you for your email.

I have got further details from our secondary antibody expert. They have advised that the light chain of secondary antibody causes significant cross reactivity between species so they recommended to use preadsorbed antib...

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Product code: 6721
Lot number: GR27131-6
Inquiry: Dear Sir or Madam unspecific binding was observed using this secondary anti-rabbit antibody (ab6721) in the cases of human serum and brain samples. It was examined without any primary antibody for ...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

- We...

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Hi Abcam,

Please see below for an email we have received from a customer;

I have received a vial of AB6721 from you and it says to store at 4°C and not to freeze. Has the composition of this antibody changed at all as we have always ...

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Thank you for your enquiry.

I could not find any information regarding a change of storage recommendation for this product.

As a general recommendation, enzyme-conjugated antibodies, such as ab6721, should not be frozen at all and should...

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Inquiry: Hi I am looking through your products of goat secondary antibodies and I found that you have 3 different types of antibodies (cat: AB97051, AB6721 and AB136636). May I know what are the difference between these products besides the price and amou...

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Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they wer...

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I have purchased 2 antibodies for my work from abcam

1) primary: Rabbit polyclonal antibody to lipocalin 2, ab 41105,
2) secondary: Goat polyclonal antibody to rabbit HRP conjugated, ab6721,
3) I am using DAB from sigma .

H...

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Thank you for contacting us. I am sorry that these antibodies are not giving any signal in IHC.

Have you been able to use the secondary antibody successfully with any other primaries? If not, I would recommend spotting the secondary and DAB reag...

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Good Afternoon,
I recently placed an order for a replacement antibody. I received this conformation email (see below) and I just wanted to make sure that we will not be charged for the antibody. Despite the fact that email says we will not there seem...

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Thanks for your enquiry.

Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order. I...

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attempting to stain mouse intestine via IHC-P; this tissue definitely expresses villin and is a pos control sample; another antibodyhas been used to stain this same tissue successfully; primary used at 1:1000 and no staining

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

Estimated delivery date of this replacement vial is August 27 2012. To check...

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I contact you because I have ordered several antibodies (proforma 41977) and I have a problem for one of them: the item ab6721 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)).
Just after reception I put the antibody in the freezer -...

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Thank you for contacting us. i apologize for any inconvenience.

We are aware of this issue; the brown precipitates as observed are very common with HRP conjugated secondary antibodies so our lab is devising ways to get rid of these precipitates h...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"