Goat Anti-Rabbit IgG Fc (ab97196)
Key features and details
- Goat Anti-Rabbit IgG Fc
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG Fc
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Immunogen
Recombinant fragment corresponding to Rabbit IgG.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads . -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97196 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000)
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WB |
1/1000 - 1/30000. (Colorimetric: 1/1000 - 10000; Chemiluminescent: 1/1000 - 1/30000)
|
Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/30000) |
WB
1/1000 - 1/30000. (Colorimetric: 1/1000 - 10000; Chemiluminescent: 1/1000 - 1/30000) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97196 has been referenced in 2 publications.
- Shu J et al. Mechanism of MicroRNA-375 Promoter Methylation in Promoting Ovarian Cancer Cell Malignancy. Technol Cancer Res Treat 20:1533033820980115 (2021). PubMed: 33928819
- Xu H et al. Downregulation of microRNA-429 protects cardiomyocytes against hypoxia-induced apoptosis by increasing Notch1 expression. Int J Mol Med 37:1677-85 (2016). PubMed: 27082497