Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/2500.
1/100 - 1/500.
1/10000 - 1/200000. (Primary ELISA)
1/10000 - 1/200000. (Chemiluminescent)
References for Goat Anti-Rabbit IgG Fc (Biotin) (ab97198)
This product has been referenced in:
Lee JR et al. Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus. Sci Rep6:27623 (2016).
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