Goat Anti-Rabbit IgG Fc (Biotin) (ab97198)
Key features and details
- Goat Anti-Rabbit IgG Fc (Biotin)
- Conjugation: Biotin
- Host species: Goat
- Isotype: IgG
- Suitable for: IHC-P, ICC/IF, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG Fc (Biotin)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG. -
Tested applications
Suitable for: IHC-P, ICC/IF, ELISA, WBmore details -
Conjugation
Biotin
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells. -
Conjugation notes
Biotin/ antibody protein ratio is 4:1 -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/250 - 1/2500.
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ICC/IF |
1/100 - 1/500.
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ELISA |
1/10000 - 1/200000. (Primary ELISA)
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|
WB |
1/10000 - 1/200000. (Chemiluminescent)
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Notes |
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IHC-P
1/250 - 1/2500. |
ICC/IF
1/100 - 1/500. |
ELISA
1/10000 - 1/200000. (Primary ELISA) |
WB
1/10000 - 1/200000. (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab97198 has been referenced in 2 publications.
- Ko SY et al. Cancer-derived small extracellular vesicles promote angiogenesis by heparin-bound, bevacizumab-insensitive VEGF, independent of vesicle uptake. Commun Biol 2:386 (2019). PubMed: 31646189
- Lee JR et al. Multiplex giant magnetoresistive biosensor microarrays identify interferon-associated autoantibodies in systemic lupus erythematosus. Sci Rep 6:27623 (2016). PubMed: 27279139