Overview

  • Product nameGoat Anti-Rabbit IgG Fc (HRP)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - Fc (HRP)
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG.
  • Tested applicationsICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary ELISA)
WB 1/2000 - 1/25000. (Colorimetric: 1/2000 - 1/20000; Chemiluminescent: 1/5000 - 1/25000)

Goat Anti-Rabbit IgG Fc (HRP) images

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97200, 1/200 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Ab97200 was used at dilution 1/5000 with the primary antibody ab90045 in WB. See the review on ab90045.

References for Goat Anti-Rabbit IgG Fc (HRP) (ab97200)

This product has been referenced in:
  • Chen M  et al. Low-dose X-ray irradiation promotes osteoblast proliferation, differentiation and fracture healing. PLoS One 9:e104016 (2014). WB . Read more (PubMed: 25089831) »
  • Peng CW  et al. Combined features based on MT1-MMP expression, CD11b + immunocytes density and LNR predict clinical outcomes of gastric cancer. J Transl Med 11:153 (2013). Read more (PubMed: 23787075) »

See all 5 Publications for this product

Product Wall


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
http://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hr...

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Unserem Wissen nach wurde ab7778 bisher noch nicht inSchwein getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zurzeit ein spezielles Angebot...

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Thank youf or your inquiry.

We have many goat anti rabbit IgG HRP conjugated antibodies:

Please use the links below to review the datasheets of three of them:

ab97051

http://www.abcam.com/index.html?datasheet=970...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not...

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We have been informed by the source of the secondary antibodies ab97195 and ab97200 that : By immunoelectrophoresis and ELISA, ab97195 (anti-Rabbit IgM - HRP) reacts specifically with rabbit IgM. This antibody may cross react with IgM from other spec...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"