Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed (ab27237)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (20nm Gold), pre-adsorbed
  • Target speciesRabbit
  • Tested applicationsElectron Microscopymore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Rabbit IgG

  • ConjugationGold 20nm

Properties

Applications

Our Abpromise guarantee covers the use of ab27237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed images

    • ab27237 used in Electron Microscopy.
      Subcellular localization of RIG-I in human neutrophils.

      C: Isolated peripheral blood neutrophils were fixed, washed with PBS, permeabilized, and blocked (10% normal goat serum, 10 mm glycine, and 0.2% Tween 20 in diluent containing 0.5% bovine serum albumin and 0.5% Triton X-100 in PBS). For visualization of RIG-I, the grids were incubated with anti-RIG-I antibody and a gold-conjugated secondary antibody (gold sphere diameter, 5 nm; ab27237, Abcam). Finally, the grids were stained with 1% uranyl acetate. Negative controls were obtained by omitting the primary antibody.

      Arrows indicate RIG-I staining. The red arrows mark RIG-I associated with a vesicle-like structure.

    References for Goat Anti-Rabbit IgG H&L (20nm Gold) preadsorbed (ab27237)

    This product has been referenced in:
    • Berger M  et al. Neutrophils Express Distinct RNA Receptors in a Non-canonical Way. J Biol Chem 287:19409-17 (2012). Electron Microscopy . Read more (PubMed: 22532562) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"