Goat Anti-Rabbit IgG H&L (5nm Gold) preadsorbed (ab27235)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (5nm Gold) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Gold 5nm), pre-adsorbed
  • Target speciesRabbit
  • Tested applicationsElectron Microscopymore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    Full length native Rabbit IgG

  • ConjugationGold 5nm

Properties

Applications

Our Abpromise guarantee covers the use of ab27235 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Electron Microscopy
  • Application notesElectron Microscopy: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Rabbit IgG H&L (5nm Gold) preadsorbed (ab27235)

    This product has been referenced in:
    • Obermayer A  et al. New aspects on the structure of neutrophil extracellular traps from chronic obstructive pulmonary disease and in vitro generation. PLoS One 9:e97784 (2014). Electron Microscopy . Read more (PubMed: 24831032) »
    • Gault WJ  et al. Drosophila CK1-?, gilgamesh, controls PCP-mediated morphogenesis through regulation of vesicle trafficking. J Cell Biol 196:605-21 (2012). Read more (PubMed: 22391037) »

    See all 4 Publications for this product

    Product Wall

    The 5nm and 10nm are differentiable, but we recommend using a bigger gap between the 2 sizes – for example a 5nm and 15 or 20nm – the size difference between these products when viewed under TEM is much clearer.

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    The antibody is directly adsorbed onto the surface of the gold nanoparticle so there is no linker present to speak of. It is known that the average length of an IgG antibody is ˜7nm so the gold label will be ˜7nm a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"