Goat Anti-Rabbit IgG H&L (ab97047)
Key features and details
- Goat Anti-Rabbit IgG H&L
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/200 - 1/2000.
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ELISA |
1/100 - 1/30000. ELISA-Coating: 1:100 - 1:500; ELISA-Primary 1:1000 - 1:30,000
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WB |
1/1000 - 1/30000. WB-Colorimetric 1:1,000 - 1:10,000; WB-Chemiluminescent 1:1,000 - 1:30,000
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/200 - 1/2000. |
ELISA
1/100 - 1/30000. ELISA-Coating: 1:100 - 1:500; ELISA-Primary 1:1000 - 1:30,000 |
WB
1/1000 - 1/30000. WB-Colorimetric 1:1,000 - 1:10,000; WB-Chemiluminescent 1:1,000 - 1:30,000 |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (15)
ab97047 has been referenced in 15 publications.
- Jafari A et al. Exosomes derived from human placental mesenchymal stem cells in combination with hyperbaric oxygen synergically alleviates spinal cord ischemia-reperfusion injury. Regen Ther 24:407-416 (2023). PubMed: 37727798
- Ghezelayagh Z et al. The effect of mTOR activation and PTEN inhibition on human primordial follicle activation in ovarian tissue culture. J Assist Reprod Genet 39:1739-1747 (2022). PubMed: 35819576
- Johansson PA et al. A cis-acting structural variation at the ZNF558 locus controls a gene regulatory network in human brain development. Cell Stem Cell 29:52-69.e8 (2022). PubMed: 34624206
- Dong X et al. Major Vault Protein (MVP) Associated With BRAFV600E Mutation Is an Immune Microenvironment-Related Biomarker Promoting the Progression of Papillary Thyroid Cancer via MAPK/ERK and PI3K/AKT Pathways. Front Cell Dev Biol 9:688370 (2021). PubMed: 35433709
- Li S et al. Long Non-Coding RNA CAR10 Facilitates Non-Small Cell Lung Cancer Cell Migration and Invasion by Modulating the miR-892a/GJB2 Pathway. Cancer Manag Res 13:1967-1979 (2021). PubMed: 33664589