Goat Anti-Rabbit IgG H&L (Agarose) (ab7019)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Agarose)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, IPmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Agarose

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.2
    Preservative: 0.1% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product is an IgG fraction antibody coupled to activated agarose.
  • Conjugation notes
    Coupled to cyanogen bromide activated cross-linked 4% agarose. 10.0 mg antibody per cc agarose
  • Clonality
    Polyclonal
  • General notes


    Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies and proteins, including recombinant fusion proteins, from a complex protein mixture (see protocol). Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure rabbit IgG.
AP Use at an assay dependent dilution.

References for Goat Anti-Rabbit IgG H&L (Agarose) (ab7019)

ab7019 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your enquiry and patience. Usually when a reverse image is seen, it is due to too much HRP in the system and it is suggested to dilute the HRP-conjugate at least 10-fold. It could also be due to the antibody settling over time, so that wh...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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