Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 488)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)
  • Target speciesRabbit
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, ELISA, IHC-Frmore details
  • ConjugationAlexa Fluor® 488. Ex: 495nm, Em: 519nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark.
  • Storage bufferPreservative: 0.02% Sodium azide
    Constituents: PBS, 30% Glycerol, 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab150077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/200 - 1/1000.
Flow Cyt 1/2000 - 1/4000.

ab199091 - Rabbit monoclonal IgG (Alexa Fluor® 488), is suitable for use as an isotype control to complement this secondary antibody.

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) images

  • ICC/IF image of beta Tubulin staining in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab6046, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab150077 Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

  • IHC - Wholemount of Caenorhabditis elegans larvae labelling RNA polymerase II CTD repeat YSPTSPS (phospho S2) with ab5095. The sample was incubated with primary antibody (1/500 in PBS + 3% BSA + 0.1% Triton X-100) for 12 hours at 4°C. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/1000), was used as the secondary antibody.

    See Abreview

  • The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) and (ab16048, 1µg/ml) overnight at +4°C. The secondary antibodies were ab150115 Alexa Fluor® 647 (red) goat anti-mouse IgG (H+L) used at 2µg/ml for 1h and ab150077 Alexa Fluor® 488 (green) goat anti-rabbit IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei.

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used at 1/4000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Overlay histogram showing HeLa cells stained with ab32356 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32356, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) at 1/2000 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10^6 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References for Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

This product has been referenced in:
  • Li CX  et al. 'Obligate' anaerobic Salmonella strain YB1 suppresses liver tumor growth and metastasis in nude mice. Oncol Lett 13:177-183 (2017). Read more (PubMed: 28123538) »
  • Liu J  et al. Homemade-device-induced negative pressure promotes wound healing more efficiently than VSD-induced positive pressure by regulating inflammation, proliferation and remodeling. Int J Mol Med 39:879-888 (2017). Read more (PubMed: 28290607) »

See all 39 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Been using this for a while. Very happy with the results!
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Sebastian Alvarado

Verified customer

Submitted Dec 09 2015

Application Immunohistochemistry (Frozen sections)
Primary antibody anti-Glycine (ab9442) was used at 1 in 500. Secondary antibody Goat anti-Rabbit (ab150077) was used at 1 in 1000, for 2 hours at room temperature. Following 3 washes there was still strong staining and little background noise.
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Abcam user community

Verified customer

Submitted May 23 2014

Application Immunocytochemistry/ Immunofluorescence
Nice bright, clear staining observed with secondary antibody (ab150077) at a 1:200 dilution. The primary antibody used was to Human Nanog (ab21624).
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Dr. Sarah Ritson

Verified customer

Submitted Oct 07 2013

Application Immunocytochemistry/ Immunofluorescence
Following fixation in 4% PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200. The results show that nuclear Sall4 (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here).
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Abcam user community

Verified customer

Submitted Aug 21 2013

Application Immunocytochemistry/ Immunofluorescence
Cells were fixed in 4% PFA, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT then incubated with ab124297 at a 1/100 dilution or Rabbit IgG Isotype control for 2 hours at RT. The secondary used was a Goat polyclonal Secondary Antibody to Rabbit IgG – H&L Alexa Fluor 488 (ab150077) used at 1/200 dilution.
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Joe Segal

Verified customer

Submitted Aug 16 2013

The LAMP-1 antibody ab24170 is an excellent choice for staining this lysosome marker, and the anti- rabbit IgG ab150077 is the correct secondary antibody.

The antibody raised in donkey, ab150073, would also work, and as far as we know there ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"