Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab6722)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ELISA, Dot blot, ICC/IF, IHC-Fr, ICCmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    Alkaline Phosphatase

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 50% Glycerol, 10mg/ml BSA, 0.15M Sodium chloride, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 8.0
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Alkaline Phosphatase (Calf Intestine) (Molecular Weight 140,000 daltons)
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6722 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
WB 1/1000 - 1/4000.
ELISA 1/3000 - 1/5000.
Dot blot Use at an assay dependent dilution.
ICC/IF 1/200 - 1/1000.
IHC-Fr Use at an assay dependent dilution.
ICC Use at an assay dependent dilution.

Images

  • Ab6722 was used at dilution 1/10000 with the primary antibody ab16731 in WB. See the review on ab16731.
  • Ab6722 was used with the primary antibody ab2594 in ELISA. See Abreview on ab2594.

References

This product has been referenced in:
  • Liu CT  et al. Production of interleukin-4 in CD133+ cervical cancer stem cells promotes resistance to apoptosis and initiates tumor growth. Mol Med Rep 13:5068-76 (2016). WB . Read more (PubMed: 27121303) »
  • Wang G  et al. Upregulation of miR-34a by diallyl disulfide suppresses invasion and induces apoptosis in SGC-7901 cells through inhibition of the PI3K/Akt signaling pathway. Oncol Lett 11:2661-2667 (2016). Read more (PubMed: 27073535) »

See all 14 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Just as with a common indirect ELISA it is important to stop the reaction using the appropriate stop solution.

I hope this information is helpful to you. Please do not hesitate to contact us if you...

Read More

I am not aware of any cross reactivity of this antibody with human Ig. However we do offer a number of secondary antibodies which are designed to reduce background levels by either being pre-absorbed against other proteins and Igs or by digestion into ...

Read More

Thank you for contacting me again. It is always a pleasure to hear from you.

After reviewing your email, I am lead to believe that this really is a hook effect as you had mentioned. I am not an expert on this hook effect in plasma specifical...

Read More

Thank you for contacting us, I am glad to hear that the new product is working well for you.

Usually when color is developing slowly it is one of three issues. 1) the plates and reagents have not come to room temperature. 2) the conjugates a...

Read More

Thank you for contacting Abcam.

Enzymatic detection with an antibody that has been conjugated to HRP or to AP is a widely used and robust method. As this target is located on the peroxisome membrane I would recommend using anAlkaline Phospha...

Read More

Thank you for your reply. I have sent a free vial of ab34767 to replace ab28664. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. To check the status of the order please contact our Cu...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up