Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/100 - 1/1000.
ELISA 1/5000 - 1/50000. Primary
WB 1/5000 - 1/50000. Chemiluminescent detection

Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate 
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/5000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/20000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/50000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 4 minutes

References for Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)

This product has been referenced in:
  • Jeong KI  et al. CX3CR1 Is Expressed in Differentiated Human Ciliated Airway Cells and Co-Localizes with Respiratory Syncytial Virus on Cilia in a G Protein-Dependent Manner. PLoS One 10:e0130517 (2015). WB ; Human . Read more (PubMed: 26107373) »
  • Mehrbod P  et al. Simvastatin modulates cellular components in influenza A virus-infected cells. Int J Mol Med 34:61-73 (2014). WB . Read more (PubMed: 24788303) »

See all 4 Publications for this product

Product Wall

MVH

Excellent
Abreviews
Application Western blot
30ug of oocyte lysate with RIPA
primary1:1000
12hrs 4deg
Secondary 1:2000
1hr room temp
detection with alkaline phosphatase substrate
Username

Abcam user community

Verified customer

Submitted Jul 06 2016

Thank you for contacting us and your interest in our products.

The Anti-Osteopontin antibody (ab8448) and the Goat anti-Rabbit IgG H&L (AP) secondary antibody (ab97048) should be suitable for the experiment you are planning. They have bot...

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Thank you for your inquiry. I am happy to confirm that all these products are suitable for WB and tested and guaranteed.
I suggest ab29889 (Liver (Human) Tissue Lysate - adult normal tissue) as positive control for ab3366, ab3373, ab3375 and ab241...

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Thank you for contacting us.


As the secondary Goat anti-Rabbitantibody was raised against the whole heavy and light chains of theIgG molecule, and as a Fab2 fragment contains both as well, I don't see way the antibody shouldn't react. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"