Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) (ab97048)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase)
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG and with light chains common to other rabbit immunoglobulins. No antibody was detected against non- immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 1.19% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride, 0.00136% Zinc chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Conjugation notes
Molar enzyme: antibody protein ratio is 1:1 -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/5000 - 1/50000. Primary
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WB | (1) |
1/5000 - 1/50000. Chemiluminescent detection
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/5000 - 1/50000. Primary |
WB
1/5000 - 1/50000. Chemiluminescent detection |
Images
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RSV mutants show expected patterns of G, F, and M2-1 expression.
Western blotting of Vero cell lysates infected with RSV ΔG, ΔG C-term, and wild type (MSA1). (Figure A) Detection of G using anti-G polyclonal antibody. (Figure B) Detection of F using anti-F antibody. (Figure C) Detection of M2-1 using anti-M2-1 antibody. Each Figure shows samples that were run on the same gel and irrelevant lanes are covered with white rectangles. Molecular weights in kD are shown in Figure A.
Cell lysates from virus cultures grown for 6 days on Vero cells were subjected to SDS-PAGE, transferred to membranes and probed with a proprietary anti-RSV F antibody or anti-M2-1 antibody (Abcam, ab94805), and incubated with a secondary goat anti-mouse alkaline phosphatase conjugate. Anti-G western blot was done with rabbit anti-RSV-G polyclonal and incubated with goat anti-rabbit secondary antibody (Abcam, ab97048). Bands were visualized using BCIP/NBT. The molecular weight marker used was Precision Plus Protein Dual Color Standards.
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All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) whole cell lysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AP) (ab97048) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 4 minutes
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab97048 has been referenced in 39 publications.
- Jacques LC et al. Influence of Streptococcus pneumoniae Within-Strain Population Diversity on Virulence and Pathogenesis. Microbiol Spectr 11:e0310322 (2023). PubMed: 36507681
- Tomaszewska E et al. Repetitive Cerulein-Induced Chronic Pancreatitis in Growing Pigs-A Pilot Study. Int J Mol Sci 24:N/A (2023). PubMed: 37175426
- Tomaszewska E et al. Prenatal Fumonisin Exposure Impairs Bone Development via Disturbances in the OC/Leptin and RANKL/RANK/OPG Systems in Weaned Rat Offspring. Int J Mol Sci 24:N/A (2023). PubMed: 37240089
- Wilton DK et al. Microglia and complement mediate early corticostriatal synapse loss and cognitive dysfunction in Huntington's disease. Nat Med 29:2866-2884 (2023). PubMed: 37814059
- Liu K et al. GRP75 as a functional element of cholix transcytosis. Tissue Barriers 11:2039003 (2023). PubMed: 35262466