Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed (ab97072)
Key features and details
- Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
- Conjugation: Alkaline Phosphatase
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, IHC-P, ELISA, WB
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
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Tested applications
Suitable for: ICC, IHC-P, ELISA, WBmore details -
Minimal
cross-reactivity
Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details -
Conjugation
Alkaline Phosphatase
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.00136% Zinc chloride, 0.595% HEPES, 0.2% BSA, 0.58% Sodium chloride, 0.0095% Magnesium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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IHC-P |
1/100 - 1/1000.
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ELISA |
1/2500 - 1/25000. (Primary)
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WB |
1/2500 - 1/25000. (Chemiluminescent)
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Notes |
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ICC
Use at an assay dependent dilution. |
IHC-P
1/100 - 1/1000. |
ELISA
1/2500 - 1/25000. (Primary) |
WB
1/2500 - 1/25000. (Chemiluminescent) |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab97072 has been referenced in 2 publications.
- Wang N et al. The introduction of an N-glycosylation site into prochymosin greatly enhances its production and secretion by Pichia pastoris. Microb Cell Fact 21:177 (2022). PubMed: 36042512
- Krstic Ristivojevic M et al. Alpha-Gal on the Protein Surface Hampers Transcytosis through the Caco-2 Monolayer. Int J Mol Sci 21:N/A (2020). PubMed: 32796496