Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (AMCA)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, Flow Cyt, IHC-Frmore details
  • Immunogen

    Other Immunogen Type corresponding to IgG.

  • Conjugation
    AMCA. Ex: 350nm, Em: 445nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    pH: 7.20
    Preservative: 0.013% Sodium azide
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Conjugation notes
    Blue fluorescence. Excitation/Emission wavelength= 344 nm/ 446 nm
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 30 µg/ml.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 26720273

Images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained stained with DRAQ7TM (ab109202)

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is ...

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Unfortunately we do not havesecondary antibodies conjugated to Alexa 405 or Pacific Blue.
However, we have the following secondary antibodies, conjugated to AMCA, a blue fluorescence, Excitation/Emission wavele...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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