Goat Anti-Rabbit IgG H&L (AMCA) (ab123435)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferpH: 7.20
    Preservative: 0.013% Sodium azide
    Constituents: 99% Phosphate Buffer, 0.88% Sodium chloride
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesBlue fluorescence. Excitation/Emission wavelength= 344 nm/ 446 nm
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab123435 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 - 30 µg/ml.
WB Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (AMCA) images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rat anti-EndoA alpha 1, 1:100
    Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody: Goat polyclonal Secondary Antibody to Rat IgG - H&L (Cy3®) (ab98416), 1:100
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained with SYTO 16

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

    Preparation:

    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness


    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (AMCA) (ab123435), 1:100
    Nuclei were counterstained stained with DRAQ7TM (ab109202)

References for Goat Anti-Rabbit IgG H&L (AMCA) (ab123435)

ab123435 has not yet been referenced specifically in any publications.

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Unfortunately we do not havesecondary antibodies conjugated to Alexa 405 or Pacific Blue.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"