Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)

Overview

• Product name
  Goat Anti-Rabbit IgG H&L (Biotin)
  See all IgG secondary antibodies
• Target species
  Rabbit
• Tested applications
  Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-Fr, IHC-Pmore details
• Immunogen

  Rabbit IgG whole molecule

• Conjugation
  Biotin

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  pH: 7.20
  Preservative: 0.01% Sodium azide
  Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
• Concentration information loading...
• Purity
  IgG fraction
• Purification notes
  This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Conjugation notes
  Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

  • Secondary antibodies
  • anti-Rabbit
  • IgG
  • Biotin

Goat Anti-Rabbit IgG H&L (Biotin) images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)

  IHC image of beta actin staining in a section of formalin-fixed paraffin-embedded [human normal colon]*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated with ab8227, 1/1000 dilution, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody (ab6720, 1/1000 dilution) was used to detect the primary, and visualized using an HRP conjugated ABC system. Streptavidin HRP was used, ab7403 at a 1/10000 dilution. DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (Biotin) (ab6720)This image is courtesy of an anonymous Abreview.

  ab21156 staining pancreatic amylase in mouse pancreas tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 4% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG H&L (ab6720) (1/2000) was used as the secondary antibody.

  See Abreview

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit IgG antibody - H&L (ab6720)This image is courtesy of an anonymous Abreview
  ab28829 (Rabbit polyclonal to STAT6 (phospho Y641)) at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

  See Abreview

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  Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) (ab6720)
  Ab6720 was used at dilution 1/100 with the primary antibody ab15000 in IHC-Fr. See the review on ab15000.