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Our Abpromise guarantee covers the use of ab97049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/5000.|
|ICC/IF||1/200 - 1/1000.|
|ELISA||1/20000 - 1/400000. ELISA-Primary|
|WB||1/20000 - 1/400000. WB-Chemiluminescent|
Ab97049 was used at dilution 1:500 for IHC analysis of GATA4 expression in paraformaldehyde fixed, paraffin embedded testis sections. The primary antibody was a rabbit polyclonal IgG raised against human GATA4.
Image on the left: isotype control image
This image is courtesy of Dr Kyle Caires