Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab97073)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Biotin) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97073 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250 - 1/2500.
ICC/IF 1/100 - 1/500.
ELISA 1/10000 - 1/200000. (Primary)
WB 1/10000 - 1/200000. (Chemiluminescent)

References for Goat Anti-Rabbit IgG H&L (Biotin) preadsorbed (ab97073)

ab97073 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"