Goat Anti-Rabbit IgG H&L (Chromeo™ 488) (ab60314)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Chromeo™ 488)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Specificityab60314 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IFmore details
  • Immunogen

    Rabbit IgG.

  • ConjugationChromeo™ 488

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesChromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets. Molar Extinction Coefficient: 73,000 M-1cm-1 (measured at Amax) Quantum Yield: ~ 52 % Excitation Wavelength Range: 470 to 500 nm Emission Wavelength Range: 515 to 530 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/2000.
Plate-based assays: 1/800 - 1/1000.

Goat Anti-Rabbit IgG H&L (Chromeo™ 488) images

  • ICC/IF imaging of anti-tubulin in U2OS cells stained with propidium iodide and incubated with rabbit anti-tubulin antibody. Ab60314 was then used at 1:1000. The cells were previously fixed and permeabilized by methanol.

  • Ab60314 was used at dilution 1/500 with the primary antibody ab32787 in IHC-P. See the review on ab32787.

References for Goat Anti-Rabbit IgG H&L (Chromeo™ 488) (ab60314)

This product has been referenced in:
  • Watcharin W  et al. Biodegradable human serum albumin nanoparticles as contrast agents for the detection of hepatocellular carcinoma by magnetic resonance imaging. Eur J Pharm Biopharm N/A:N/A (2013). Read more (PubMed: 24365328) »
  • Oh TJ  et al. Effect of the combination of metformin and fenofibrate on glucose homeostasis in diabetic Goto-Kakizaki rats. Exp Mol Med 45:e30 (2013). ICC/IF . Read more (PubMed: 23827952) »

See all 3 Publications for this product

Product Wall

We store the antibody at 4°C . At the concentration of 2 mg/ml the conjugate is stable for a long period of time, though the duration of stability is unknown. We tested antibodies after more than two years in the fridge and did not recognize a diff...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry these secondary antibodies did not perform as stated on the datasheet and for the inco...

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Thank you for taking the time to complete our questionnaire. The details you have kindly provided will provide us with vital information for our monitoring of product quality

I appreciate the time you have spent in the laboratory and underst...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondary antibodies.

I appreciate the time you have spent in the laboratory and understand your concerns. ...

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As requested, I have issued a free of charge replacement with the order number XXXXX for the antibody ab60314.

To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vial...

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Thank you for contacting us.

The fluorochromes which are sold under the brand names Alexa, DyLight or Chromeo are designed to ensure an optimal performance and thus are chemically almost identical. As you may have noticed the advantagesfor th...

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I think its good that you are checking the cell viability and using HeLa may provide more information as to if it is the cell strain or the antibodies that may be contributing to the problems. I agree that checking both the primary and secondary antibo...

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Thank you for providing such a full explanation of what you are trying to achieve. I can now understand exactly why you have chosen the technique that you have.

I think the experiments you are planning to do next will be very useful in determ...

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Thank you for getting back to me with your new results. It is good that we can now say conclusively that there is non-specific binding of both the secondary antibody and of the isotype control antibody.

Following the protocol changes, I do no...

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Thank you for that last bit of information.


I just wanted to check that the isotype control wasn't being used in a higher concentration than the primary antibody, but everything is fine.


I look forward to hearing how you...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"