Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Chromeo™ 546)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Specificityab60317 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applicationsICC/IFmore details
  • Immunogen

    Rabbit IgG.

  • ConjugationChromeo™ 546

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Conjugation notesChromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo™ 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 13 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab60317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notesICC/IF: 1/1000 - 1/2000.
    Plate-based assays: 1/800 to 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Rabbit IgG H&L (Chromeo™ 546) (ab60317)

    This product has been referenced in:
    • Hoppe S  et al. Rapid identification of novel immunodominant proteins and characterization of a specific linear epitope of Campylobacter jejuni. PLoS One 8:e65837 (2013). Read more (PubMed: 23734261) »
    • Strobel S  et al. Insectivorous bats digest chitin in the stomach using acidic mammalian chitinase. PLoS One 8:e72770 (2013). Read more (PubMed: 24019876) »

    See all 4 Publications for this product

    Product Wall

    There are currently no Abreviews or Questions for ab60317.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"