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Product name Goat Anti-Rabbit IgG H&L (Chromeo™ 546) See all IgG secondary antibodies
Target species Rabbit
Specificity ab60317 reacts with whole molecular rabbit IgG and the light
chains of other rabbit immunoglobulins. No cross-reactivity with
non-immunoglobulin serum proteins observed.
Tested applications Suitable for:
ICC/IF more details
Conjugation Chromeo™ 546 Properties
Storage instructions Shipped at 4°C. Store at +4°C.
Storage buffer Preservative: 2mM Sodium Azide
Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
Concentration information loading...
Purity Immunogen affinity purified
Conjugation notes Chromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo™ 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets.
Molar extinction Coefficient: 96,800 M -1cm -1 (measured at Amax)
Quantum Yield: ~ 13 %
Excitation Wavelength Range: 530 to 550 nm
Emission Wavelength Range: 560 to 570 nm
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes ICC/IF: 1/1000 - 1/2000.
Plate-based assays: 1/800 to 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
References for Goat Anti-Rabbit IgG H&L (Chromeo™ 546) (ab60317)
This product has been referenced in:
et al. Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PLoS One 9:e110703 (2014).
Read more (PubMed: 25333280) »
et al. Rapid identification of novel immunodominant proteins and characterization of a specific linear epitope of Campylobacter jejuni. PLoS One 8:e65837 (2013).
Read more (PubMed: 23734261) »
See all 5 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"