Conjugation notesChromeo™ 488 is spectraly similar to FITC and Alexa® 488. Conjugated
antibodies exhibit superior luminescent properties and stability towards
photobleaching, and have absorption and emission maxima of approximately 490 and 527 nm which are compatible with common excitation sources and filter sets.
Molar Extinction Coefficient: 73,000 M-1cm-1 (measured at Amax)
Quantum Yield: ~ 52 %
Excitation Wavelength Range: 470 to 500 nm
Emission Wavelength Range: 515 to 530 nm
ICC/IF imaging of anti-tubulin in U2OS cells stained with propidium iodide and incubated with rabbit anti-tubulin antibody. Ab60314 was then used at 1:1000. The cells were previously fixed and permeabilized by methanol.
Ab60314 was used at dilution 1/500 with the primary antibody ab32787 in IHC-P. See the review on ab32787.
References for Goat Anti-Rabbit IgG H&L (Chromeo™ 488) (ab60314)
This product has been referenced in:
Watcharin W et al. Biodegradable human serum albumin nanoparticles as contrast agents for the detection of hepatocellular carcinoma by magnetic resonance imaging. Eur J Pharm BiopharmN/A:N/A (2013).
Read more (PubMed: 24365328) »
Oh TJ et al. Effect of the combination of metformin and fenofibrate on glucose homeostasis in diabetic Goto-Kakizaki rats. Exp Mol Med45:e30 (2013).
Read more (PubMed: 23827952) »