Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Chromeo™ 546)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    ab60317 reacts with whole molecular rabbit IgG and the light chains of other rabbit immunoglobulins. No cross-reactivity with non-immunoglobulin serum proteins observed.
  • Tested applications
    Suitable for: ICC/IFmore details
  • Immunogen

    Rabbit IgG.

  • Conjugation
    Chromeo™ 546

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 2mM Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.01M Potassium phosphate, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Conjugation notes
    Chromeo™ 546 is spectrally similar to Cy3®. Conjugated antibodies exhibit superior luminescent properties and stability towards photobleaching. Chromeo™ 546 conjugated antibodies have absorption and emission maxima of approximately 545 and 568 nm which are compatible with common excitation sources and filter sets. Molar extinction Coefficient: 96,800 M-1cm-1 (measured at Amax) Quantum Yield: ~ 13 % Excitation Wavelength Range: 530 to 550 nm Emission Wavelength Range: 560 to 570 nm
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab60317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: 1/1000 - 1/2000.
    Plate-based assays: 1/800 to 1/1000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • References for Goat Anti-Rabbit IgG H&L (Chromeo™ 546) (ab60317)

    This product has been referenced in:

    See all 6 Publications for this product

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