Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)
Key features and details
- Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
- Conjugation: Cy2 ®. Ex: 489nm, Em: 505nm
- Host species: Goat
- Isotype: IgG
- Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISA
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details -
Minimal
cross-reactivity
Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details -
Immunogen
Full length native Rabbit IgG (purified).
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Conjugation
Cy2 ®. Ex: 489nm, Em: 505nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Tripotassium orthophosphate, 0.87% Sodium chloride, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. -
Conjugation notes
Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Cy™ and CyDye™ are registered trademarks of Cytiva.
This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.
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Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution.
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ICC/IF |
1/1000 - 1/5000.
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IHC-P |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent dilution.
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ELISA |
1/10000 - 1/50000.
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Notes |
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WB
Use at an assay dependent dilution. |
ICC/IF
1/1000 - 1/5000. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent dilution. |
ELISA
1/10000 - 1/50000. |
Images
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Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution + Rabbit IgG at 0.05 µg
Incubated with ab6940 for 1 hour at room temperature.
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All lanes : Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940) at 1/1000 dilution
Lane 1 : Rabbit IgG whole molecule
Lane 2 : Rabbit IgG F(ab) fragment
Lane 3 : Rabbit IgG F(c) fragment
Lane 4 : Rabbit IgM whole molecule
Lane 5 : Normal rabbit serum
Lysates/proteins at 0.05 µg per lane.
Secondary
All lanes : Peroxidase-conjugated donkey anti-goat IgG at 1/40000 dilution
Performed under reducing conditions.
Observed band size: 23,25,50,70 kDa why is the actual band size different from the predicted?Incubated with the primary antibody for 1 hour at room temperature.
Incubated with the secondary antibody for 30 minutes at room temperature.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (39)
ab6940 has been referenced in 39 publications.
- Obi AT et al. Experimental venous thrombus resolution is driven by IL-6 mediated monocyte actions. Sci Rep 13:3253 (2023). PubMed: 36828892
- Tian Q et al. Gut dysbiosis contributes to chlamydial induction of hydrosalpinx in the upper genital tract. Front Microbiol 14:1142283 (2023). PubMed: 37125189
- Mitamura R et al. NEAT1_2 and DAZAP1, Paraspeckle Components, Interact with PXR to Negatively Regulate CYP3A4 Induction. Drug Metab Dispos 51:1230-1237 (2023). PubMed: 37349114
- Chen L et al. Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis. Theranostics 13:3550-3567 (2023). PubMed: 37441598
- Deng R et al. Downregulation of TUSC3 promotes EMT and hepatocellular carcinoma progression through LIPC/AKT axis. J Transl Med 20:485 (2022). PubMed: 36274132