Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy2 ®), pre-adsorbed
  • Target species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IHC-Fr, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    Cy2 ®. Ex: 489nm, Em: 505nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • Conjugation notes
    Cy2 (Cyanine 2-OSu) (Molecular Weight 897 daltons) Absorption Wavelength: 489 nm Emission Wavelength: 505 nm Fluorochrome/Protein Ratio: 2.0 moles Cy2 per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labeled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ELISA 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed images

  • ab6940 was used at dilution 1/10000 with the primary antibody ab47840 in WB. See the review on ab47840.

References for Goat Anti-Rabbit IgG H&L (Cy2 ®) preadsorbed (ab6940)

This product has been referenced in:
  • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). ICC/IF . Read more (PubMed: 27611779) »
  • Admoni-Elisha L  et al. Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment. PLoS One 11:e0148500 (2016). Read more (PubMed: 27078856) »

See all 2 Publications for this product

Product Wall

Application
Immunocytochemistry
We have used this secondary antibody for immunocytochemistry with 2,2'-thiodiethanol (TDE) mounting media. TDE quenches the fluorescence of conventional green fluorophores (e.g., Alexafluor 488, GFP, etc....), but the original description of this mounting media (PMID: 17131355) reported an increase in the relative quantum yield of Cy3. We tried this Cy2-conjugated secondary antibody following TDE mounting of stained HEK293 cells and saw excellent signal from the green channel. We did not observe bleaching of the signal following Z-stack image acquisition.

The protocol for obtaining the image shown in the figure was a standard ICC protocol, followed by the TDE mounting procedure from the original TDE paper (PMID: 17131355), using sequential immersions in increasing concentrations of TDE. Slides were imaged on a Zeiss Observer Z1 with LED-based excitation (488 nm). Acquired images were deconvolved and the included image is a maximum intensity projection.
Username

Dr. Sam Nowitzki

Verified customer

Submitted Jul 09 2015

Thank you for contacting us.

Yes, either ab6940 or ab97075 will work as a suitable secondary with ab7779. "Pre-adsorbed" means that we pre-treated the secondary antibody with normal sera from certain species to remove cross-reactiv...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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