Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed
  • Target speciesRabbit
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • Conjugation notesCy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesThis product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/100. PubMed: 17045908
ICC Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed images

  • Ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

References for Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

This product has been referenced in:
  • Islam R  et al. Effect of Storage Temperature on Structure and Function of Cultured Human Oral Keratinocytes. PLoS One 10:e0128306 (2015). ICC ; Human . Read more (PubMed: 26052937) »
  • Buchholz M  et al. A multistep high-content screening approach to identify novel functionally relevant target genes in pancreatic cancer. PLoS One 10:e0122946 (2015). ICC . Read more (PubMed: 25849100) »

See all 20 Publications for this product

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