Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed
  • Target species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, IHC-P, ICC, Flow Cyt, ELISAmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • Conjugation
    Cy3 ®. Ex: 552nm, Em: 565nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum.
  • Conjugation notes
    Cy3.29 (Cyanine 3.29-OSu) (Molecular Weight 949 daltons) Absorption Wavelength: 552 nm Emission Wavelength: 565 nm Fluorochrome/Protein Ratio: 9.9 moles Cy3 per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited. This material is also subject to proprietary rights of GE Healthcare Bio-Sciences Corp. and Carnegie Mellon University and made and sold under License from GE Healthcare Bio-Sciences Corp. This product is licensed for sale only for research. It is NOT licensed for any other use. There is no implied license hereunder for any commercial use. COMMERCIAL USE shall include: 1 Sale, lease, license or other transfer of the material or any material derived or produced from it. 2 Sale, lease, license or other grant of rights to use this material or any material derived or produced from it. 3 Use of this material to perform services for a fee for third parties. If you require a commercial license to use this material and do not have one, please return this material, unopened to Abcam Plc of 330 Cambridge Science Park, Cambridge, CB4 0FL, and any money paid for the material will be refunded.


    This secondary antibody is specifically designed for the detection of multiple primary antibodies (polyclonal or monoclonal) of different host species in experiments where cells are simultaneously labelled without unwanted cross reaction.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6939 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-P 1/100. PubMed: 17045908
ICC Use at an assay dependent dilution.
Flow Cyt 1/500 - 1/2500.
ELISA 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed images

  • Ab6939 was used at dilution 1/500 with the primary antibody ab10812 in ICC/IF. See the review on ab10812.

References for Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab6939)

This product has been referenced in:
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). ICC/IF . Read more (PubMed: 28158775) »
  • Yan J  et al. Herbal medicine Yinchenhaotang protects against a-naphthylisothiocyanate-induced cholestasis in rats. Sci Rep 7:4211 (2017). WB, IF ; Rat . Read more (PubMed: 28646179) »

See all 32 Publications for this product

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It is clear from the images provided that the customer has observed some background with the secondary antibody. In order to reduce this, I would advise reducing the concentration which is currently being use...

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