Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab97075)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3 ®), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG.

  • ConjugationCy3 ®. Ex: 552nm, Em: 565nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97075 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.

Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)

     

    Preparation:
    Fix in 3%PFA in PBS for 30 min at RT
    Incubate in 7.5% sucrose-PBS for 3h at RT
    Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    Embed the EBs in tissue-Tek OCT compound
    Cut frozen sections to 4-20 µm thickness

     

    Primary antibody: Rabbit anti-laminin alpha 1, 1:400

     

    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3®) (ab97075), 1:100
    Nuclei were counterstained with SYTO 16

     

     

  • Image: Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

    Sample: mouse endoderm cell

    Preparation:

    Fix in 3% PFA in PBS for 30 min at RT

    Primary antibody: Rabbit anti-talin (ab71333), 1:100

    Secondary antibody: Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy3® 488) preadsorbed (ab97075), 1:100

References for Goat Anti-Rabbit IgG H&L (Cy3 ®) preadsorbed (ab97075)

ab97075 has not yet been referenced specifically in any publications.

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1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; http:...

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Thank you for contacting Abcam and for your interest in our products.


All three of the secondary antibodies you have suggested are compatible with the primary antibody ab7779. In terms of the color of the staining, you can expect the foll...

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Thank you for contacting us.

Yes, either ab6940 or ab97075 will work as a suitable secondary with ab7779. "Pre-adsorbed" means that we pre-treated the secondary antibody with normal sera from certain species to remove cross-reactiv...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"