Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)


  • Product nameGoat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5 ®), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Pmore details
  • Minimal

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationCy5 ®. Ex: 650nm, Em: 667nm


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab97077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
Flow Cyt 1/100 - 1/1000.
IHC-P 1/50 - 1/500.

Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed images

  • Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)


    • Fix in 3% PFA in PBS for 30 min at RT
    • Incubate in 7.5% sucrose-PBS for 3h at RT
    • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
    • Embed the EBs in tissue-Tek OCT compound
    • Cut frozen sections to 4-20 µm thickness

    Primary antibody: Rabbit anti-laminin alpha 1, 1:400
    Secondary antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Cy5®) (ab97077), 1:100
    F-actin was stained with CytoPainter F-actin staining kit (blue) (ab112124), 1:100
    Nuclei were counterstained with SYTO 16

  • ab97077 (1/200) detecting ab5176 in assynchronous HeLa cells (red). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.

References for Goat Anti-Rabbit IgG H&L (Cy5 ®) preadsorbed (ab97077)

This product has been referenced in:
  • Arslan E  et al. Glycosaminoglycan-mimetic signals direct the osteo/chondrogenic differentiation of mesenchymal stem cells in a three-dimensional peptide nanofiber extracellular matrix mimetic environment. Biomacromolecules N/A:N/A (2016). Read more (PubMed: 26840042) »
  • Regot S  et al. High-sensitivity measurements of multiple kinase activities in live single cells. Cell 157:1724-34 (2014). ICC/IF . Read more (PubMed: 24949979) »

See all 4 Publications for this product

Product Wall

Our most recent lot of this has a labeling (F/P) ratio of 6, that is, six Cy5 molecules per antibody molecule.

Thank you for your response.

ab97077 is a goat anti-rabbit secondary so it is unlikely to react with ab91493 (host species hamster). However, ab97037 is goat anti-mouse secondary antibody and it may cross-react with hamster IgG since mouse an...

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Thank you for your enquiry.

Could you please confirm if the customer would like to perform double or triple staining? I am a bit confused why the two selected secondary antibodies ie ab97037 and ab97077 (Cy5 ®) have the same fluorophore? ...

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