Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)


  • Product name
    Goat Anti-Rabbit IgG H&L (DyLight® 488)
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488)
  • Target species
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Immunogen

    Other Immunogen Type corresponding to Rabbit IgG.

  • Conjugation
    DyLight® 488. Ex: 493nm, Em: 518nm


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab96883 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rabbit IgG H&L (DyLight® 488) images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rabbit IgG H&L (DyLight® 488) (ab96883)

This product has been referenced in:
  • Decourtye L  et al. IGF-1 Induces GHRH Neuronal Axon Elongation during Early Postnatal Life in Mice. PLoS One 12:e0170083 (2017). Read more (PubMed: 28076448) »
  • Cen M  et al. TIEG1 deficiency confers enhanced myocardial protection in the infarcted heart by mediating the Pten/Akt signalling pathway. Int J Mol Med 39:569-578 (2017). Read more (PubMed: 28204828) »

See all 8 Publications for this product

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
ab18956 staining GDNF in rat brain tissue sections by IHC-P (Formalin/PFA-fixed paraffin-embedded sections). Cells were fixed with paraformaledhyde, permeabilized with 0.1% Tween-20 and PBS and blocked with 1% BSA for 30 minutes at 25°C antigen retrieval was by heat mediation in a citrate buffer pH 6.0. Samples were incubated with primary antibody (1/150 in TBS) for 24 hours at 4°C. ab96883 at a dilution of 1/200 was used as the secondary antibody.
( Green = GDNF, Blue = DAPI stained nuclei.)

Abcam user community

Verified customer

Submitted Dec 03 2015

Goat anti-Rabbit IgG H&L (DyLight® 488) is good to use.

Abcam user community

Verified customer

Submitted Aug 07 2015

Thank you for your enquiry.

Could you confirm that Ki67 primary antibody you are referring to has product code ab16667?

I can confirm that for ab16667, ab125938 would be a suitable isotype control, and ab96883 would be a suitable ...

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Thank you for your enquiry.

Here are the answers to your quesitons.

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