Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96895)


  • Product nameGoat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to human, mouse and rat IgG was detected.
  • Tested applicationsFlow Cyt, ICC/IF, IHC-P, WB, IHC-Frmore details
  • Minimal

    Human, Mouse, Rat more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm


  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas


Our Abpromise guarantee covers the use of ab96895 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50 - 1/200.
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500.
WB 1/1000 - 1/20000.
IHC-Fr 1/200.

following abreview ab96895

Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed images

  • Overlay histogram showing Jurkat cells stained with ab16669 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab16669, 1/1000 dilution) for 30 min at 22°C. The secondary antibody Goat anti-rabbit IgG H&L (DyLight® 488, pre-adsorbed) (ab96895) was used at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
  • Glial fibrillary acidic protein(GFAP) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 488), pre-adsorbed (ab96895) was used as a secondary antibdoy at a dilution of 1/200.
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96895)

This product has been referenced in:
  • Xu Y  et al. Pseudomonas aeruginosa pyocyanin activates NRF2-ARE-mediated transcriptional response via the ROS-EGFR-PI3K-AKT/MEK-ERK MAP kinase signaling in pulmonary epithelial cells. PLoS One 8:e72528 (2013). ICC/IF . Read more (PubMed: 24015256) »
  • Pilia M  et al. Influence of substrate curvature on osteoblast orientation and extracellular matrix deposition. J Biol Eng 7:23 (2013). ICC/IF . Read more (PubMed: 24090183) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
In combination with Goat polyclonal antibody to Rat IgG- H&L (Cy3®), ab98416, anti-CD3 antibody (ab16669) and anti-CD19 antibodies (ab25232).
•Frozen sections were fixed in 4% paramformaldehyde in PBS for 15 min, and then blocked with 10% goat serum for 30 min and washed 3 times with ice-cold PBS.
•Slides were incubated with mixture of anti-CD3 (ab16669) and anti-CD19 antibodies (ab25232), diluted 1:200 in PBST (1× PBS with 0.05% Tween-20), 1% BSA for 1 hour in a humidified chamber at room temperature and then washed 4 times in PBS (5 min each).
•Slides were incubated for 1 hour in dark at room temperature with mixture of secondary antibodies: Goat DyLight® 488-conjugated anti-Rabbit (ab96895) and Goat Cy3®-conjugated antibody to Rat IgG - H&L (ab98416). Secondary antibodies were 1:200 diluted in PBST, 1% BSA.
•Slides were washed 3 times with PBS in dark (each wash for 5 min).
•For DNA-staining, slides were counterstained with 0.1 µg/ml DAPI for 5 min and finally rinsed with PBS, left 10 minutes for air-drying in dark and finally mounted.
•CD3 antigen appears green CD19 red and the nuclei are stained blue.
•Fluorescent microscope picture at 20x.

Herr Dr. Nasr Hemdan

Verified customer

Submitted Jul 15 2013