Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)


  • Product nameGoat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal

    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationDyLight® 488. Ex: 493nm, Em: 518nm


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab96899 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/500.

Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed images

  • ICC/IF image of (ab3280) stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3280, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)

This product has been referenced in:
  • Varikmaa M  et al. Role of mitochondria-cytoskeleton interactions in respiration regulation and mitochondrial organization in striated muscles. Biochim Biophys Acta 1837:232-45 (2014). Read more (PubMed: 24189374) »
  • Han J  et al. Fuzi attenuates diabetic neuropathy in rats and protects schwann cells from apoptosis induced by high glucose. PLoS One 9:e86539 (2014). ICC/IF . Read more (PubMed: 24466139) »

See all 13 Publications for this product

Product Wall

Merci de votre intérêt pour l'anti-Mucin5AC ab78660 et le secondaire ab96899.

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Thank you for contacting us and your interest in our products. The immunogen sequence used to raise theab56215 Anti-SLC4A4 antibodyis proprietary information. However, in order to aid your research I am able to share it with you. Please do not share...

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I am very pleased to hear you would like to accept our offer and test ab28856 inIHC-Fr. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this...

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Thank you very much for your interest in 28856 & ab51059.

To our knowledge, 28856 & ab51059 have not been tested in IHC-Fr. Therefore, I can offer a discount off a future purchase if you buy 28856 & ab51059 now, test it in IHC-Fr...

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Thank you for contacting us.

The ab292 is a rabbit antibody which target human collagen I. To detect this primary you would need secondary which is ab96899.

If your primary ab works in human then the secondary ab will work.

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Thank you for contacting us.

Please buy ab96899;http://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96899.html

The Dylight 488 conjugate is better than Alexa 488.

I hope t...

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Thank you for your inquiry.

We have a number of products that are comparable with the products on the list you sent us. For future inquiriesI would like to direct you to the search facility on our website (www.abcam.com).

Please e...

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It is my pleasure to help.

In this particular group, aggrecan would fluoresce in green while collagen II would be red. We offer other dyes athttp://www.abcam.com/index.html?pageconfig=resource&rid=12956should you wish to change those col...

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Thank you for contacting us.

We presently have only 1 product against mouse aggrecan, ab36861. This is a rabbit polyclonal. In ICC and appropriate secondary would be anti-Rabbit IgG with a fluorescent tag which could be excited by your las...

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Thank you for contacting us.
To answer your questions:
1. Yes, a negative control can be ommiting the primary. Other options could be using an isotype control antibody that compares with the priamry, or the blocking peptide. There are various...

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