Goat Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96896)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (DyLight® 550) preadsorbedSee all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to human, mouse and rat IgG was detected.
  • Tested applicationsICC/IF, IHC-P, Flow Cyt more details
  • Minimal
    cross-reactivity

    Human, Mouse, Rat more details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using a human, mouse and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF 1/50 - 1/500.
IHC-P 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed images

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rabbit IgG H&L (DyLight® 550) preadsorbed (ab96896)

ab96896 has not yet been referenced specifically in any publications.

Product Wall

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Please find attached the Certificate of Compliances for the requested datasheets.

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I would be happy to generate Certificate of Compliances for the 3 products that you have, could you please provide me with the lot numbers of the aliquots that you have? Once I have these lot numbers I can gene...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"