Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (FITC)
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, Immunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISAmore details
  • Immunogen

    Rabbit IgG whole molecule

  • Conjugation
    FITC. Ex: 493nm, Em: 528nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C. Store In the Dark.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notes
    Fluorescein isothiocyanate (FITC) (Molecular Weight 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.7 moles FITC per mole of Goat IgG
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6717 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 24216136
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt 1/500 - 1/2500.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
ELISA 1/10000 - 1/50000.

Images

  • ab11320 (Rabbit polyclonal to gamma Tubulin - Centrosome Marker) at 1/400 dilution staining human neuroblastoma cells by ICC/IF. The cells were fixed with paraformaldehyde and blocked with serum and then incubated with the primary antibody for 16 hours. ab6717 was used as the secondary.

    See Abreview

  • Ab6717 was used at dilution 1/200 with the primary antibody ab36823 in IHC-P. See the review on ab36823.
  • Ab6717 was used with the primary antibody ab66506 in Flow Cyt. See Abreview on ab66506.

References

This product has been referenced in:
  • Maolake A  et al. Tumor-associated macrophages promote prostate cancer migration through activation of the CCL22-CCR4 axis. Oncotarget 8:9739-9751 (2017). Read more (PubMed: 28039457) »
  • Cohen JE  et al. An innovative biologic system for photon-powered myocardium in the ischemic heart. Sci Adv 3:e1603078 (2017). IHC ; Rat . Read more (PubMed: 28630913) »

See all 98 Publications for this product

Customer reviews and Q&As

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Mouse Osteoblast cells were grown on cover slips for 48 hrs. Then Cells were fixed with formaldehyde vapor for 10 mins. Cells were permeabilized with Triton-X 100 for 5-7mins. 3% BSA was used for blocking (1hr). 1:100 dilution of rabbit anti-vinculin antibody to mouse was added onto the cells and incubated for 1hr. Then 1:1000 dilution of anti-rabbit FITC conjugated secondary antibody was added on to the cells and incubated for 1hr. Before proceeding to each step cells were washed with PBS.
Username

Abcam user community

Verified customer

Submitted Jul 05 2013

I am sorry for the delay in getting back to you. I now have further information to share in regards to how the Anti-Histone H2B antibody [EP957Y] (ab52599) has been used in staining of fixed cells.

The image presented on the datasheet of ab525...

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Thanks for your reply. I am glad you found the information useful.

With the fixative you intend to use I do not think you will require permiabilisation. However, methanol is sometimes not suitable for some targets/antibodies. It may therefore ...

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Thank you for contacting us this morning in regards to the anti-Histone H2B antibody (ab52599) and the Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC) (ab6717).

These antibodies should work fine in combination for the experim...

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In order to dilute an antibody 1\1000, you can, for example, add 1ul for every 1000ul of diluent.

Regarding your question, If the vial of ab6717 contains 1mg at 2mg/ml, then it contains about 0.5ml of volume. To diute the entire 0.5...

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Vielen Dank für Ihre Anfrage.

Ich freue mich ihnen mitteilen zu koennen dass wir fuer den gesamten November unsere Aktion 4 fuer 3 laufen haben. Bei der Bestellung von 4 Produkten bekommen Sie das guenstigste Produkt kostenfrei.

...

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Thank you for contacting us.

We have an isotype control of goat IgG, conjugated to FITC. The catalogue number is ab37374.

Click here (or use the following: http://www.abcam.com/index.html?datasheet=37374).

This is sold ...

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Thank you for contacting us.
According to the datasheet, the this protein is not expressed in most lymphoid cell lines: B-cells, T-cells and NK cells. Therefore, I would suggest to use a T-cell line like Jurkat lysate as a negative control.
...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Thank you for your reply and for returning the vial of ab6785.

The vial of ab6717 will be arriving tomorrow on the order ***.


Please let me know if you need anything else, and have a wonderful evening.

1-10 of 23 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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