Overview

  • Product nameGoat Anti-Rabbit IgG H&L (HRP)See all IgG secondary antibodies ...
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
  • Target speciesRabbit
  • Tested applicationsIHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Frmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • General notesMany of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000.
WB 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).
Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances.
ELISA 1/120000.
Immunomicroscopy Use at an assay dependent dilution.
Dot Blot Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/1000.

Goat Anti-Rabbit IgG H&L (HRP) images


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

    This image is courtesy of an Abreview submitted by Maryna Polyakova.

    See Abreview


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Ouattara Bazoumana.

    See Abreview


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • ab5095 Immunoprecipitating RNA polymerase II in human K562 whole cell lysate. 20µg of cell lysate was incubated with primary antibody (1/1000 in TBST) and matrix (Protein A) for 12 hours at 4°C. For western blotting a HRP-conjugated goat anti-rabbit IgG H&L (ab6720) (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

  • ab3580 staining glucocorticoid receptor in mouse epididymis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with bouin's solution and blocked with 1.5% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/1000) for 14 hours at 4°C. A HRP-conjugated goat anti-rabbit IgG H&L (ab6720) (1/200) was used as the secondary antibody.

    See Abreview

  • Ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370.
  • Ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.

References for Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

This product has been referenced in:
  • Gasperin BG  et al. Expression of receptors for BMP15 is differentially regulated in dominant and subordinate follicles during follicle deviation in cattle. Anim Reprod Sci 144:72-8 (2014). Read more (PubMed: 24388700) »
  • Westerbeck JW  et al. A SUMO-targeted ubiquitin ligase is involved in the degradation of the nuclear pool of the SUMO E3 ligase Siz1. Mol Biol Cell 25:1-16 (2014). WB . Read more (PubMed: 24196836) »

See all 85 Publications for this product

Product Wall

Storage conditions are extremely important for the proper functioning of the antibody, so we strongly recommend that you follow the storage instructions on the datasheet for the antibody you purchased. These conditions will vary among our antibodies, t...

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Thank you for your enquiry regarding ab6721.

The dilution factor on the datasheet only a recommendation for starting. The actual dilutions need to be optimized by the scientist since it depends on many factors such expression levels of the ta...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

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I have got further details from our secondary antibody expert. They have advised that the light chain of secondary antibody causes significant cross reactivity between species so they recommended to use preadsorbed an...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

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Thank you for your enquiry.

I could not find any information regarding a change of storage recommendation for this product.

As a general recommendation, enzyme-conjugated antibodies, such as ab6721, should not be frozen at all and sho...

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Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they ...

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Thank you for contacting us. I am sorry that these antibodies are not giving any signal in IHC.

Have you been able to use the secondary antibody successfully with any other primaries? If not, I would recommend spotting the secondary and DAB r...

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Thanks for your enquiry.

Sorry for the confusion. I just wanted to confirm with you that this replacement order, is indeed a free of charge replacement. When the order was first placed the "100%" discount was not taken off the order...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

Estimated delivery date of this replacement vial is August 27 2012. To ch...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"