Overview

  • Product nameGoat Anti-Rabbit IgG H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WB more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) images

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

This product has been referenced in:
  • Lopez M  et al. Tumor necrosis factor and transforming growth factor ß regulate clock genes by controlling the expression of the cold inducible RNA-binding protein (CIRBP). J Biol Chem 289:2736-44 (2014). Read more (PubMed: 24337574) »
  • Steel JJ  et al. Subgenomic reporter RNA system for detection of alphavirus infection in mosquitoes. PLoS One 8:e84930 (2013). WB . Read more (PubMed: 24367703) »

See all 3 Publications for this product

Product Wall

This product has been tested using ECL and will perform very well using this detection system.


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
http://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hr...

Read More

Thank you for contacting us. The recommended dilution for ELISA is actually for use as a secondary antibody. We are working to correct this typo. Please let me know if you have any furuther questions.

Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they ...

Read More

Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

Read More

Thank you for contacting us again.

I apologize for forgetting your request to a suitable secondary antibody!

The product I would like to suggest is the following:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L...

Read More

Thank you for contacting us.

We currently do not offer products which meet each of the requirements that you have set. I assembled for you well characterized products which meet most of these requirements but each has some deficiency. Pric...

Read More

Thank you for contacting us.

THE MSDS can be downloaded from the top right hand corner of the datasheet. Please let me know if you are unable to find it.

I hope this information is helpful to you. Please do not hesitate to contact us ...

Read More



I would like to suggest ab97051 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)

http://www.abcam.com/index.html?datasheet=97051 (or use the following: http://www.abcam.com/index.html?datasheet=97051).

It bel...

Read More

Thanks so much for taking the time to complete this survey. I am glad to hear the replacement antibody is working as expected. If you have any questions, please do not hesitate to contact us.

Best of luck in your research.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"