Overview

  • Product nameGoat Anti-Rabbit IgG H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) images


  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Secondary
    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

This product has been referenced in:
  • Lopez M  et al. Tumor necrosis factor and transforming growth factor ß regulate clock genes by controlling the expression of the cold inducible RNA-binding protein (CIRBP). J Biol Chem 289:2736-44 (2014). Read more (PubMed: 24337574) »
  • Bhattacharyya S  et al. Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri. PLoS One 9:e89026 (2014). Read more (PubMed: 24586486) »

See all 18 Publications for this product

Product Wall

Application Western blot
Secondary worked very well. Used at 1:5000.
Username

Abcam user community

Verified customer

Submitted Aug 15 2014

This product has been tested using ECL and will perform very well using this detection system.


I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:
http://www.abcam.com/goat-polyclonal-secondary-antibody-to-rabbit-igg-hl-hr...

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Thank you for contacting us. The recommended dilution for ELISA is actually for use as a secondary antibody. We are working to correct this typo. Please let me know if you have any furuther questions.

Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they ...

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us again.

I apologize for forgetting your request to a suitable secondary antibody!

The product I would like to suggest is the following:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L...

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Thank you for contacting us.

We currently do not offer products which meet each of the requirements that you have set. I assembled for you well characterized products which meet most of these requirements but each has some deficiency. Pric...

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Thank you for contacting us.

THE MSDS can be downloaded from the top right hand corner of the datasheet. Please let me know if you are unable to find it.

I hope this information is helpful to you. Please do not hesitate to contact us ...

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I would like to suggest ab97051 Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)

http://www.abcam.com/index.html?datasheet=97051 (or use the following: http://www.abcam.com/index.html?datasheet=97051).

It bel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"