• Product nameGoat Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research areas


Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) images

  • Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

This product has been referenced in:
  • Sillaste G  et al. A novel hypothesis for histone-to-protamine transition in Bos taurus spermatozoa. Reproduction 153:241-251 (2017). WB . Read more (PubMed: 27899719) »
  • Wang Q  et al. miR-219-5p suppresses the proliferation and invasion of colorectal cancer cells by targeting calcyphosin. Oncol Lett 13:1319-1324 (2017). WB . Read more (PubMed: 28454255) »

See all 47 Publications for this product

Product Wall

Gracias por tu respuesta. Me alegra oír que este secundario os ha dado buenos resultados.

Ya he realizado por tanto el nuevo pedido completamente gratuito del mismo anticuerpo: ab97051.

Recibiréis una confirmació...

Read More

Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?

Read More
Application Western blot
this antibody works stably at a dilution of 1:4,000 for western blot assay.

Abcam user community

Verified customer

Submitted Feb 08 2016

Application Western blot
always works for WB at a dilution of 1:5,000.

Abcam user community

Verified customer

Submitted Nov 02 2015

Application Western blot
We stock and use this for many of our westerns, gets the job done!
("B" and "G" are brain and gonad samples.)

Sebastian Alvarado

Verified customer

Submitted Aug 21 2015

Application Western blot
1. The concentration of Goat Anti-Rabbit IgG H&L (HRP) used for this experiment is 1:5000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.

Mr. Lenin Veeraval

Verified customer

Submitted Jun 18 2015

Application Western blot
Abcam's anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the "epic rabbit antibody" to distinguish it from the old one we have in the lab. The lower band in the gel corresponds to beta-tubulin (ab6046) followed by anti-rabbit HRP (ab97051) while the upper band is a different 70kDa protein followed by anti-mouse HRP (ab97023).

Abcam user community

Verified customer

Submitted Aug 21 2014

Application Western blot
Secondary worked very well. Used at 1:5000.

Abcam user community

Verified customer

Submitted Aug 15 2014

This product has been tested using ECL and will perform very well using this detection system.

I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:

Read More

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