• Product nameGoat Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applicationsICC, IHC-P, ELISA, WBmore details
  • ConjugationHRP


  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas


Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000

Goat Anti-Rabbit IgG H&L (HRP) images

  • developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview.

    See Abreview

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml

    Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lysates/proteins at 10 µg per lane.

    Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/2000 dilution
    Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 10 seconds

References for Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

This product has been referenced in:
  • Sinha K  et al. Morin protects gastric mucosa from nonsteroidal anti-inflammatory drug, indomethacin induced inflammatory damage and apoptosis by modulating NF-?B pathway. Biochim Biophys Acta 1850:769-783 (2015). Read more (PubMed: 25603542) »
  • Brandt JZ  et al. Indole-3-carbinol attenuates the deleterious gestational effects of bisphenol A exposure on the prostate gland of male F1 rats. Reprod Toxicol 43:56-66 (2014). Read more (PubMed: 24257414) »

See all 21 Publications for this product

Product Wall

Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?

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Application Western blot
Abcam's anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the "epic rabbit antibody" to distinguish it from the old one we have in the lab. The lower band in the gel corresponds to beta-tubulin (ab6046) followed by anti-rabbit HRP (ab97051) while the upper band is a different 70kDa protein followed by anti-mouse HRP (ab97023).

Abcam user community

Verified customer

Submitted Aug 21 2014

Application Western blot
Secondary worked very well. Used at 1:5000.

Abcam user community

Verified customer

Submitted Aug 15 2014

This product has been tested using ECL and will perform very well using this detection system.

I would actually recommend an alternative secondary antibody ab97051, which is part of our AbExcel series of secondary antibodies. The link for ab97051 is given below:

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Thank you for contacting us. The recommended dilution for ELISA is actually for use as a secondary antibody. We are working to correct this typo. Please let me know if you have any furuther questions.

Thank you for contacting Abcam.

The main difference in the 3 secondary antibodies you mentioned are in the applications that they are tested and guaranteed to work in for 6 months as stated in our AbPromise guarantee, the method in which they ...

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Thank you for your reply. I recommend the following HRP conjugated secondary antibodies to use with these rabbit Anti-GAD65+GAD67 primary antibodies. I have included one product from a donkey host and another from goat. Both are guaranteed and the c...

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Thank you for contacting us again.

I apologize for forgetting your request to a suitable secondary antibody!

The product I would like to suggest is the following:

Goat polyclonal Secondary Antibody to Rabbit IgG - H&L...

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Thank you for contacting us.

We currently do not offer products which meet each of the requirements that you have set. I assembled for you well characterized products which meet most of these requirements but each has some deficiency. Pric...

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