Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, Immunomicroscopy, Dot blot, IHC-Frmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Full length native Rabbit IgG (purified).

  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab7090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ELISA 1/20000 - 1/80000.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed images

  • ab9986 staining osteoprotegerin in human skin tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA and 5% normal goat serum in PBS for 30 minutes at 22°C; Samples were incubated with primary antibody (1/20) for 9 hours at 4°C. An HRP-conjugated goat anti-rabbit IgG H&L (ab7090) (1/300) was used as the secondary antibody.

    See Abreview

References for Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab7090)

This product has been referenced in:
  • Esbona K  et al. COX-2 modulates mammary tumor progression in response to collagen density. Breast Cancer Res 18:35 (2016). IHC-P . Read more (PubMed: 27000374) »
  • Gu L  et al. Rapamycin ameliorates CCl4-induced liver fibrosis in mice through reciprocal regulation of the Th17/Treg cell balance. Mol Med Rep 14:1153-61 (2016). IHC-P . Read more (PubMed: 27315465) »

See all 5 Publications for this product

Product Wall

Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
This is an example of immunochemistry anti-Inos (ab15323) with Goat anti Rabbit HRP (on human artery)
-Deparaffinize
-heat in citrate buffer 20min at 96°C
-H2O2 for 10min at room temperature
-Block with PBS BSA 1% goat serum 5% at least 1h at room temperature
-incubate anti INOS at 1/100 in PBS BSA Overnight at 4°C
-incubate with goat anti rabbit HRP (1/300) for 45min at 37°C
-incubate slides in DAB or other peroxidase substrate
-counterstain with hematoxylin, dehydrate and mount
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Abcam user community

Verified customer

Submitted Jan 29 2014

Thank you for contacting us.

The Goat anti-rabbit HPR Conjugated used in AB80436 has been developed specifically for use in this kit and is currently not available for separate purchase. We do offer a number of Goat anti-rabbit secondaries...

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Thank you for your reply.

Reviewing the details, if you obtaining a band at 75 kDa in the no primary control with many secondary antibodies with the rat kidney lysate, but not the other samples, then I would recommend there is something in t...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these two secondaryantibody.

The details you have kindly provided will enable us to investigate this case for you and...

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This antibody has been pre-adsorbed against Bovine, Chicken, Goat, Guinea Pig, Horse, Human, Mouse, Rat and Sheep Serum Proteins. This minimises the cross reactivity of this antibody with these proteins. However, minimal cross reactivity will still be ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"