Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

Overview

  • Product nameGoat Anti-Rabbit IgG H&L (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat IgG was detected.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Chicken, Cow, Horse, Human, Mouse, Pig, Rat more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross absorbed using bovine, chicken, horse, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. as primary.
WB 1/5000 - 1/50000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000.

Goat Anti-Rabbit IgG H&L (HRP) preadsorbed images

  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97080, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution developed using the ECL technique, with ab87342.

References for Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080)

This product has been referenced in:
  • Brüsehafer K  et al. Chromosome breakage induced by the genotoxic agents mitomycin C and cytosine arabinoside is concentration and p53 dependent. Toxicol Sci 140:94-102 (2014). Read more (PubMed: 24675086) »
  • Weil AF  et al. Uracil DNA glycosylase initiates degradation of HIV-1 cDNA containing misincorporated dUTP and prevents viral integration. Proc Natl Acad Sci U S A 110:E448-57 (2013). Read more (PubMed: 23341616) »

See all 3 Publications for this product

Product Wall

Pré-adsorption est une étape supplémentaire pour augmenter la spécificité d'un anticorps. Je vous envoie un lien pour notre site internet qui explique ce terme en plus de détailes.

http://www.abcam.c...

Read More

Thank you for contacting us.

Please have a look at the troubleshooting guide given on our website;

http://www.abcam.com/index.html?pageconfig=resource&rid=11384

I am sorry I am unable to get the specific protocol for chic...

Read More

Thank you for your reply.

While I did not receive the image which you have referenced I would like to offer so help regarding your questions. Generally specific details regarding secondary antibody incubation and concentration as will need...

Read More

Thank you for contacting us.

Abcam takes product quality seriously and we recently undertook an in-depth study examining storage conditions and antibody performance. After over 13,000 individual ELISA experiments, we have determined that sto...

Read More

Thank you for your email.
I'm glad to see that the antibody worked.
Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
http://www.abcam.com/abreviews

Thank you for your email.
I'd suggest starting with the recommended dilutions for each antibody.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products...

Read More

Thank you for your email.
The free of charge order was shipped today for delivery tomorrow.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Use our products? Sub...

Read More

You are very welcome. I keep my fingers crossed for you and wish you good luck.
Yes, definitely, please keep me informed. I look forward to hear back from you - hopefully with positive results.
Have a nice weekend.

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

Read More

Thank you for your email.
Yes, I'd be happy to send you 1 vial of each ab6721 and ab97051.
Could you please let me know your order or PO number or order date for the 2 vials of ab97080 so that I can locate this in our system?
Thank you s...

Read More

1-10 of 21 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"