Overview

  • Product nameGoat Anti-Rabbit IgG H&L (TRITC)
    See all IgG secondary antibodies
  • Target speciesRabbit
  • Tested applicationsSuitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details
  • Immunogen

    Rabbit IgG, whole molecule

  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
  • Conjugation notesTetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.3 moles TRITC per mole of Goat IgG
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Immunomicroscopy Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent dilution.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ELISA 1/10000 - 1/50000.

References for Goat Anti-Rabbit IgG H&L (TRITC) (ab6718)

This product has been referenced in:
  • Ganapathy K  et al. Influence of 6-Hydroxydopamine Toxicity on a-Synuclein Phosphorylation, Resting Vesicle Expression, and Vesicular Dopamine Release. J Cell Biochem 117:2719-2736 (2016). Read more (PubMed: 27064513) »
  • Zavala-Zendejas VE  et al. Claudin-6, 7, or 9 overexpression in the human gastric adenocarcinoma cell line AGS increases its invasiveness, migration, and proliferation rate. Cancer Invest 29:1-11 (2011). IF . Read more (PubMed: 20874001) »

See all 6 Publications for this product

Product Wall

Application Immunomicroscopy
Goat anti-Rabbit IgG H&L (TRITC) is good to label iNOS in mouse hepatocytes.
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Abcam user community

Verified customer

Submitted Aug 07 2015

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

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I am sorry hear about the disappointing results. If customer have purchased the secondary antibody from Abcam (ab6718) and if the problem is due to secondary antibody then I am happy to replace it for this customer.
Please let me know the order num...

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Thank you for providing details.
This is strange, if the red cells are seen even without primary antibody then the problem is either due to secondary antibody or due to protocol. I have checked the protocol and I find no problem with it other than ...

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Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.
I have read the details you have kindly provided and have following further questions for better understanding of the problem;
- What was ...

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Thank you for your reply.

I am very sorry to hear you are still experiencing problems with these products. According to the images sent it appears the secondary is still giving a lot of non specific background.

If the antibody is st...

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Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would appreciate if you could please send me the order details, so I can check there isn’t any particular issue...

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Thank you for your enquiry. 1) Ab27089 is supplier as a pre diluted product and Ab4055 is not. 2) We do not have specific feedback in relation to certain secondary Abs functioning in conjunction with our antibodies; however a list of compatible s...

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Thank you for your enquiry. According to the datasheet this antibody is in liquid form (the buffer is: 0.02M K Phosphate, 0.15M NaCl, 0.01% Sodium Azide, pH7.2) so you do not need to reconstitute it. The unit size is 1 mg and the concentration is 2 mg/...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"