Overview

  • Product nameGoat Anti-Rabbit IgG H&L (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRabbit
  • SpecificityThis antibody binds all rabbit Igs. The antibody preparation does not react with human IgG by immunoelectrophoresis.
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    IgG isolated from pooled normal rabbit serum

  • Positive controlHuman peripheral blood lymphocytes and human tonsil tissue.
  • ConjugationTRITC. Ex: 547nm, Em: 572nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrior to conjugation, the antibody preparation is solid phase adsorbed with human IgG to minimize reactivity with human tissues or cell preparations.
  • Conjugation notesAb50598 is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notes


    F/P Molar Ratio: 1.0-5.0 Protein Concentration: 3.0-6.0 mg/ml by absorbance at 280 nm.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/160.
ICC/IF 1/400.

References for Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed (ab50598)

This product has been referenced in:
  • Bara JJ  et al. Three-dimensional culture and characterization of mononuclear cells from human bone marrow. Cytotherapy 17:458-72 (2015). IHC . Read more (PubMed: 25680302) »
  • Loibl M  et al. Direct cell-cell contact between mesenchymal stem cells and endothelial progenitor cells induces a pericyte-like phenotype in vitro. Biomed Res Int 2014:395781 (2014). ICC/IF . Read more (PubMed: 24563864) »

See all 4 Publications for this product

Product Wall

There are currently no Abreviews or Questions for ab50598.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"