Overview

  • Product name
    Goat Anti-Rabbit IgG H&L (TRITC) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Rabbit
  • Specificity
    This antibody binds all rabbit Igs. The antibody preparation does not react with human IgG by immunoelectrophoresis.
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Minimal
    cross-reactivity

    Human more details
  • Immunogen

    IgG isolated from pooled normal rabbit serum

  • Positive control
    Human peripheral blood lymphocytes and human tonsil tissue.
  • Conjugation
    TRITC. Ex: 547nm, Em: 572nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Prior to conjugation, the antibody preparation is solid phase adsorbed with human IgG to minimize reactivity with human tissues or cell preparations.
  • Conjugation notes
    Ab50598 is conjugated to crystalline tetramethylrhodamine isothiocyanate (TRITC) and then further purified to remove free TRITC.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes


    F/P Molar Ratio: 1.0-5.0 Protein Concentration: 3.0-6.0 mg/ml by absorbance at 280 nm.

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab50598 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/160.
ICC/IF 1/400.

References

This product has been referenced in:
  • Ghezzi CE  et al. 3D Functional Corneal Stromal Tissue Equivalent Based on Corneal Stromal Stem Cells and Multi-Layered Silk Film Architecture. PLoS One 12:e0169504 (2017). Read more (PubMed: 28099503) »
  • Leckelt J  et al. Early detection of diabetic neuropathy by investigating CNFL and IENFD in thy1-YFP mice. J Endocrinol 231:147-157 (2016). Read more (PubMed: 27601446) »

See all 6 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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