Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

Overview

  • Product nameGoat Anti-Rat IgG Fc (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Human, Mouse more details
  • ConjugationDyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • ClonalityPolyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed images

  • Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.
  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

ab96972 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Immunocytochemistry/ Immunofluorescence
yellow channel is anti-Rat Dy550, label a cell surface protein, no permeablization. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h
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Ms. YA ZHOU

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"