Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

Overview

  • Product name
    Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed
    See all IgG secondary antibodies
  • Target species
    Rat
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • Tested applications
    Suitable for: IHC-P, ICC/IF, Flow Cytmore details
  • Minimal
    cross-reactivity

    Human, Mouse more details
  • Conjugation
    DyLight® 550. Ex: 562nm, Em: 576nm

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • General notes
    DyLight® 550 replaces DyLight® 549.
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/500.
ICC/IF 1/50 - 1/500.
Flow Cyt 1/50 - 1/200.

Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed images

  • Anti dopamine transporter (DAT) staining in mouse Formalin Fixed Paraffin Embedded sections. For antigen retrieval HIER in citrate buffer was performed. Goat polyclonal Secondary Antibody to Rat IgG - Fc (DyLight® 550), pre-adsorbed (ab96972) was used as a secondary antibody at a concentration of 1/500.
  • Immunocytochemsitry/Immunofluorescence analysis of HEK293 cells using Goat Anti-Rat IgG Fc (DyLight® 550) preabsorbed (ab96972) in yellow labelling a cell surface protein. Cells were incubated with the secondary antibody (1/500 in PBS + 2% BSA) for 1 hour at room temperature.

  • Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Goat Anti-Rat IgG Fc (DyLight® 550) preadsorbed (ab96972)

ab96972 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Immunocytochemistry/ Immunofluorescence
yellow channel is anti-Rat Dy550, label a cell surface protein, no permeablization. work at dilution of 1:500, incubate in 2%BSA/PBS at RT for 1h
Username

Ms. Ya Zhou

Verified customer

Submitted Feb 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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