Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090)

Overview

  • Product nameGoat Anti-Rat IgG Fc (HRP) preadsorbed
    See all IgG secondary antibodies
  • Description
    Goat polyclonal Secondary Antibody to Rat IgG - Fc (HRP), pre-adsorbed
  • Target speciesRat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was dtected against IgA, IgM or non-immunoglobulin serum proteins. Less than 0.1% cross reactivity to human and mouse IgG was detected.
  • Tested applicationsSuitable for: ICC, IHC-P, ELISA, WBmore details
  • Minimal
    cross-reactivity

    Human, Mouse more details
  • ConjugationHRP

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity.This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme: antibody protein ratio is 1:1
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/2000.
ELISA 1/10000 - 1/100000. (Primary)
WB 1/2000 - 1/25000. Colorimetric: use 1/2000 -1 /20000, Chemiluminescent: use 1/5000 - 1/25000

References for Goat Anti-Rat IgG Fc (HRP) preadsorbed (ab97090)

ab97090 has not yet been referenced specifically in any publications.

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The datasheet of these antibodies say "Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles"

This mean after receiving the...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"