Goat Anti-Rat IgG Fc preadsorbed (ab97086)
Key features and details
- Goat Anti-Rat IgG Fc preadsorbed
- Host species: Goat
- Isotype: IgG
- Suitable for: ICC, ELISA, WB, IHC-P
Related conjugates and formulations
Overview
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Product name
Goat Anti-Rat IgG Fc preadsorbed
See all IgG secondary antibodies -
Host species
Goat -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rat IgG. No antibody was detected against IgA, IgM or non-immunoglobulin serum proteins. Reduced cross-reactivity to human and mouse IgG was detected.
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Tested applications
Suitable for: ICC, ELISA, WB, IHC-Pmore details -
Minimal
cross-reactivity
Human, Mouse more details
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. Antiserum was solid phase adsorbed to ensure class specificity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use at an assay dependent dilution.
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ELISA |
1/2500 - 1/25000. as primary antibody.
Use at 1/100 - 1/500 as coating antibody.
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WB |
1/1000 - 1/10000. with colorimetric detection.
Use at 1/1000 - 1/30000 with chemiluminescent detection.
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IHC-P |
1/100 - 1/1000.
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Notes |
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ICC
Use at an assay dependent dilution. |
ELISA
1/2500 - 1/25000. as primary antibody. Use at 1/100 - 1/500 as coating antibody. |
WB
1/1000 - 1/10000. with colorimetric detection. Use at 1/1000 - 1/30000 with chemiluminescent detection. |
IHC-P
1/100 - 1/1000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab97086 has been referenced in 1 publication.
- Hata J et al. Complement activation by autoantigen recognition in the growth process of benign prostatic hyperplasia. Sci Rep 9:20357 (2019). PubMed: 31889151