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Rat IgG whole molecule
Our Abpromise guarantee covers the use of ab6846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/20000.|
|Immunomicroscopy||Use at an assay dependent concentration.|
|Dot blot||Use at an assay dependent concentration.|
|ICC/IF||1/200 - 1/1000.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
All lanes : Anti-Tubulin antibody [YL1/2] - Loading Control (ab6160) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/5000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/20000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Rat IgG - H&L (AP) (ab6846) at 1/50000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 52 kDa
Additional bands at : 85 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 8 minutes
Abcam recommends blocking with 3% milk to reproduce this western blot data.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"