Overview

  • Product nameGoat Anti-Rat IgG H&L (Biotin)
    See all IgG secondary antibodies
  • Target speciesRat
  • Tested applicationsSuitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details
  • Immunogen

    Rat IgG whole molecule

  • ConjugationBiotin

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG molecule
  • ClonalityPolyclonal
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Immunomicroscopy Use at an assay dependent concentration.
Dot blot Use at an assay dependent concentration.
ICC/IF 1/1000 - 1/5000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Goat Anti-Rat IgG H&L (Biotin) images

  • Ab6844 was used at dilution 1/500 with the primary antibody ab6640 in IHC-P. See the review on ab6640.

References for Goat Anti-Rat IgG H&L (Biotin) (ab6844)

This product has been referenced in:
  • Tachibana H  et al. Activation of liver X receptor inhibits osteopontin and ameliorates diabetic nephropathy. J Am Soc Nephrol 23:1835-46 (2012). Read more (PubMed: 23085633) »
  • Cicko S  et al. Purinergic receptor inhibition prevents the development of smoke-induced lung injury and emphysema. J Immunol 185:688-97 (2010). IHC-P . Read more (PubMed: 20519655) »

See all 3 Publications for this product

Product Wall

Thank you for your equiry. This antibody has not been adsorbed against mouse. I hope this information helps. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"